antibody

Glypican 3 (Glypiated Proteoglycan 3) (PE)

MBS600821

100 Tests

670 USD

monoclonal

mouse

PE

[7H23]

flow cytometry, FACS

Antibody

Glypican 3 (Glypiated Proteoglycan 3) (PE)

[Glypican 3]

[Anti -Glypican 3 (Glypiated Proteoglycan 3) (PE)]

[glypican 3; Glypican-3; glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7]

[GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; GPC3; OCI5]

GPC3_HUMAN

Monoclonal

IgG2a

[7H23]

Mouse

Human

Recognizes human Glypican 3. No cross-reactivity with recombinant human Glypican-2, recombinant human Glypican-5 or recombinant human Glypican-6.

Purified by Protein A affinity chromatography from hybridoma culture supernatant

Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with R-PHYCOERYTHRIN (PE).

0.025mg/ml

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Use 10ul to label 1x10e6 cells. Optimal dilutions to be determined by the reseacher.

Immunogen: Recombinant protein corresponding to aa25-558 from human Glypican 3, expressed in NSO cells (P51654.1)

Grade: Affinity Purified

Antibodies; Abs to Binding Proteins

Glypicans are GPI-anchored heparan sulfate proteoglycans that include a large globular cysteine-rich domain with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Glypicans are thought to function as low-affinity binding proteins that retain signaling molecules (cytokines, growth factors, proteases) at the cell surface. Glypicans are widely expressed in adult and fetal tissues.

2599578

P51654.1

65,563 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Glycosaminoglycan Metabolism Pathway (645297); Glypican 3 Network Pathway (138084); Glypican Pathway (138083); HS-GAG Biosynthesis Pathway (645306); HS-GAG Degradation Pathway (645307); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (645304); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family. Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; anchored to plasma membrane; integral to plasma membrane; Golgi lumen; plasma membrane. Molecular Function: heparan sulfate proteoglycan binding; protein binding. Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; pathogenesis; positive regulation of endocytosis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; positive regulation of glucose import; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of growth; anterior/posterior axis specification; negative regulation of epithelial cell proliferation; lung development. Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1

100 Tests

670 USD