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Mouse Anti-Human HLA Class 1 Antigen A30,A31 (R0.87)

MBS600673

0.1 mL

440 USD

Antibody

Mouse Anti-Human HLA Class 1 Antigen A30,A31 (R0.87)

[HLA Class 1 Antigen A30,A31]

[Anti -HLA Class 1 Antigen A30,A31 (R0.87)]

[HLA-A31, partial; HLA class I histocompatibility antigen, A-68 alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; major histocompatibility complex, class I, A; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68]

[HLA-A; HLA-A; HLAA; HLAA]

1A68_HUMAN

IgM

[4i103]

Recognizes human HLA Class 1 Antigen-A30,A31. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.

Ascites

Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute to 100ul with sterile ddH2O to make a 10X stock solution.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Cytoxicity: Add 0.9 mL of PBS, 1% BSA to 100 uL of 10x Stock solution. Optimal dilutions to be determined by the researcher.

Testing Data

Immunogen: HLA Class 1 Antigen-A30, A31.

Antibodies; Abs to HLA

The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans and is an essential element for immune function. This group of genes re- sides on chromosome 6, and encodes cell-surface antigen- presenting proteins and many other genes. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell, including viral peptides if present. These peptides are produced from digested proteins that are broken down in the proteasomes. Foreign antigens attract killer T-cells (also called CD8+ or cytotoxic T-cells) that destroy cells.

386879

AAA59613.1

P01891

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83069)

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; endoplasmic reticulum; early endosome membrane; plasma membrane; MHC class I protein complex; Golgi medial cisterna. Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding. Biological Process: regulation of immune response; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; positive regulation of interferon-gamma production; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response. Disease: Severe Cutaneous Adverse Reaction, Susceptibility To

0.1 mL

440 USD