catalog number :
MBS600655
products full name :
a-Dystroglycan (Cranin)
products short name :
a-Dystroglycan
products name syn :
Anti -a-Dystroglycan (Cranin)
reactivity :
Canine, Guinea Pig, Human, Mouse, Rabbit, Rat
specificity :
Recognizes rabbit alpha-Dystroglycan at ~156kD. Species Crossreactivity: human, mouse, rat, canine and guinea pig.
purity :
Ascites. Ascites
form :
Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
ELISA (EL/EIA), Western Blot (WB)
app notes :
Suitable for use in ELISA, Inhibition of Laminin Binding to Dystroglycan and Western Blot. Dilution: Inhibition of Laminin Binding to Dystroglycan: In a nitrocellulose overlay experiment, this antibody inhibits binding of 125I Laminin to Dystroglycan. Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan in rabbit skeletal muscle. Rabbit skeletal muscle lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with D9905-05. Proteins were visualized using an IgM (HRP) goat anti-mouse and a chemiluminescence detection system. Note: The use of WGA puri ed protein results in signi cantly cleaner blots and immunoprecipitates. Post-translational modi cation of Dystroglycan causes band broadening.
other info1 :
Immunogen: Rabbit skeletal muscle membrane preparation.
products categories :
Antibodies; Abs to Carbohydrates, Glycoproteins