antibody

G9a (EHMT2, Euchromatic Histone-lysine N-methyltransferase 2)

MBS600643

0.1 ml

455 USD

monoclonal

rabbit

nonconjugated

9j132

human, mouse, rat

western blot, immunocytochemistry

Antibody

G9a (EHMT2, Euchromatic Histone-lysine N-methyltransferase 2)

G9a

Anti -G9a (EHMT2, Euchromatic Histone-lysine N-methyltransferase 2)

G9a; Histone-lysine N-methyltransferase EHMT2; histone-lysine N-methyltransferase EHMT2; protein G9a; H3-K9-HMTase 3; OTTHUMP00000029262; OTTHUMP00000029264; OTTHUMP00000174666; OTTHUMP00000174667; G9A histone methyltransferase; HLA-B associated transcript 8; HLA-B-associated transcript 8; lysine N-methyltransferase 1C; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; euchromatic histone-lysine N-methyltransferase 2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Lysine N-methyltransferase 1C; Protein G9a

EHMT2; EHMT2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30; FLJ35547; DKFZp686H08213; BAT8; C6orf30; G9A; KMT1C; NG36

EHMT2_HUMAN

Monoclonal

IgG

9j132

Host: Rabbit; Source: Human

Human, Monkey, Mouse, Rat

Recognizes endogenous levels of total human G9a (both the 165kD G9a-L and 140kD G9a-S isoforms). Does not crossreact with other histone methyl-transferases, including GLP. Species Crossreactivity

Supernatant. Supernatant.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunofluorescence (IF)

Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence (IF-IC): 1:50. Western Blot: 1:1000

Immunogen: Synthetic peptide corresponding to the carboxy terminus of human G9a (KLH).

Antibodies; Abs to Enzymes, Methyltransferase

G9a is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-di- merization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large tran- scriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and his- tone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12,13).

287865

Q96KQ7

132,370 Da

Lysine Degradation Pathway 82956!!Lysine Degradation Pathway 320!!RNA Polymerase I Promoter Clearance Pathway 106551!!RNA Polymerase I Transcription Pathway 106550!!RNA Polymerase I Transcription Initiation Pathway 106553!!RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway 160948!!Regulation Of Androgen Receptor Activity Pathway 138027!!Transcription Pathway 106549

A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq]

Function: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. Ref.8 Ref.9 Ref.17 Ref.22. Catalytic activity: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. Ref.9. Subunit structure: Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Ref.11 Ref.12 Ref.22. Subcellular location: Nucleus. Chromosome. Note: Associates with euchromatic regions. Does not associate with heterochromatin. Ref.9. Tissue specificity: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. Ref.1. Domain: The SET domain mediates interaction with WIZ. Ref.18The ANK repeats bind H3K9me1 and H3K9me2. Ref.18. Post-translational modification: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.13 Ref.14 Ref.15 Ref.16 Ref.19 Ref.20 Ref.21Methylated at Lys-185; automethylated. Ref.17. Sequence similarities: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.Contains 7 ANK repeats.Contains 1 post-SET domain.Contains 1 pre-SET domain.Contains 1 SET domain. Caution: While NG36 and G9a were originally thought to derive from 2 separate genes, all G9A transcripts also contain the in frame coding sequence of NG36 (Ref.1).It is uncertain whether Met-1 or Met-21 is the initiator methionine. Sequence caution: The sequence AAD21811.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAD21812.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAH02686.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAH09351.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAH18718.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH20970.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB63294.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB63295.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA49491.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

0.1 ml

455 USD