5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase) | MyBioSource MBS6006096 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

catalog :

MBS6006096

quantity :

0.05 mg

price :

695 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Human Placenta (FFPE) after heat-induced antigen retrieval using MBS6006096 (10ug/ml).

image 2 :

Western Blot analysis of mouse brain tissue lysate using MBS6006096.

product information

catalog number :

MBS6006096

products type :

Antibody

products full name :

5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

products short name :

[5'-Nucleotidase]

products name syn :

[Anti -5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)]

other names :

[5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; 5' nucleotidase, ecto; Ecto-5'-nucleotidase]

other gene names :

[Nt5e; Nt5e; Nt5; CD73; Nt5; Nte; 5'-NT]

uniprot entry name :

5NTD_RAT

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

specificity :

Recognizes human NT5E. Species sequence homology: Mouse.

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a liquid in PBS, 0.09% sodium azide.

concentration :

0.25 mg/ml

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Suitable for use in Western Blot, ELISA and Immunohistochemistry. Dilution: ELISA: 1:1000. Western Blot: 1:100-1:500. Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml

image1 heading :

Testing Data

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: Synthetic peptide corresponding to human NT5E at C-terminal (KLH).

products categories :

Antibodies; Abs to Enzymes, Nucleotidase

products description :

CD73 is a 70kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T- and B-cells; mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 co-stimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

ncbi gi num :

148747417

ncbi acc num :

NP_067587.2

ncbi gb acc num :

NM_021576.2

uniprot acc num :

P21588

ncbi mol weight :

63,969 Da

ncbi pathways :

Metabolism Pathway (828668); Metabolism Of Nucleotides Pathway (828815); Nicotinate And Nicotinamide Metabolism Pathway (83406); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (828819); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (828816); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (828823); Pyrimidine Metabolism Pathway (83339)

ncbi summary :

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

uniprot summary :

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; EC 3.1.3.5; Nucleotide Metabolism - purine. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane; integral to membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; ferrous iron binding; nucleotide binding. Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; positive regulation of lipid biosynthetic process; brain development; purine nucleotide biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion

size1 :

0.05 mg

price1 :

695 USD

more info or order :

MyBioSource product webpage