catalog number :
MBS600530
products full name :
HLA Class 1 Antigen B7,B27,B42 (X-Reactive)
products short name :
[HLA Class 1 Antigen B7,B27,B42]
products name syn :
[Anti -HLA Class 1 Antigen B7,B27,B42 (X-Reactive)]
specificity :
Recognizes human HLA Class 1 Antigen-B7,B27,B42. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
form :
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute to 100ul with sterile ddH2O to make a 10X stock solution.
storage stability :
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, Cytotoxicity and Chimerism Studies. Other applications have not been tested.
app notes :
Cytotoxicity: Add 900ul of 1% BSA in PBS to 100ul of 10X working dilution. Optimal dilutions to be determined by the researcher.
other info1 :
Immunogen: HLA Class 1 Antigen-B7, B27, B42. Cytotoxicity: Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
products categories :
Antibodies; Abs to HLA