catalog number :
MBS600512
products full name :
Methionine Aminopeptidase 1 (MetAP-1)
products short name :
[Methionine Aminopeptidase 1]
products name syn :
[Mouse anti-Human Methionine Aminopeptidase 1 (MetAP-1)]
other names :
[methionine aminopeptidase 1; Methionine aminopeptidase 1; methionine aminopeptidase 1; MAP 1; metAP 1; peptidase M 1; OTTHUMP00000219920; methionyl aminopeptidase 1; Peptidase M 1]
other gene names :
[METAP1; METAP1; MAP1A; MetAP1A; KIAA0094; DKFZp781C0419; KIAA0094]
uniprot entry name :
AMPM1_HUMAN
specificity :
Recognizes human Methionine Aminopeptidase 1 (MetAP-1)in direct ELISAs and Western Blot. Does not react with recombinant human METAP2.
purity :
Purified by Protein G affinity chromatography.
form :
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
concentration :
~0.5 mg/ml (after reconstitution)
storage stability :
Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA), Western Blot (WB). Other applications not tested
app notes :
Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml The detection limit is ~3ng/well. Optimal dilutions to be determined by the researcher
other info1 :
Immunogen: Recombinant protein corresponding to aa52-386 of human Methionine Aminopeptidase 1 derived from Sterile-filtered21. Species Sequence Homology: chimpanzee (99%), rat and mouse (98%), chicken (95%), drosophila (64%)
other info2 :
Grade: Affinity Purified
products categories :
Antibodies; Abs to Enzymes
products description :
Methionine Aminopeptidase 1 (MetAP-1) encodes a cytoplasmic protease that removes the NH 2-terminal Met from nascent proteins.
ncbi acc num :
NP_055958.2
ncbi gb acc num :
NM_015143.2
ncbi mol weight :
43,215 Da
uniprot summary :
METAP1: a peptidase that removes the amino-terminal methionine from nascent proteins. Protein type: Protease; EC 3.4.11.18. Chromosomal Location of Human Ortholog: 4q23. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity. Biological Process: rhodopsin mediated signaling; regulation of translation; phototransduction, visible light; regulation of rhodopsin mediated signaling; peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification