antibody

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Biotin)

MBS6003125

1 mL

600 USD

polyclonal

unidentified

biotin

human, cow

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, dot blot

Antibody

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Biotin)

[Complement C3, C3c]

[Anti -Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Biotin)]

[complement C3 preproprotein; Complement C3; complement C3; complement component 3<; C3adesArg]

[C3; C3; ASP]

CO3_BOVIN

Polyclonal

IgG

unknown

Human

Recognizes human C3c. In IEP against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of human serum or plasma.

Purified

Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Dot Blot (DB), Western Blot (WB),

Optimal dilution to be determined by the researcher.

Immunogen: C3c isolated and purified from pooled normal human serum.

Grade: Purified. Biotim/IgG protein molar ratio (B/P): ~7.1

Antibodies; Abs to Complement

C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and the extent of tissue destruction.

99028969

NP_001035559.2

NM_001040469.2

Q2UVX4

187,253 Da

Activation Of C3 And C5 Pathway (827281); Adaptive Immune System Pathway (827212); Alternative Complement Activation Pathway (827279); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Class A/1 (Rhodopsin-like Receptors) Pathway (826961); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (827274); G Alpha (i) Signalling Events Pathway (826991)

Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates . By similarity.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes . By similarity.Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2 . By similarity. Subunit structure: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). C3dg interacts with CR2 (via the N-terminal Sushi domains 1 and 2). Interacts with VSIG4. Interacts (both C3a and ASP) with C5AR2; the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of C5AR2, recruitment of ARRB2 to the cell surface and endocytosis of GRP77 . By similarity. Interacts with BHV-1 glycoprotein C. Ref.3. Subcellular location: Secreted. Post-translational modification: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by dietary chylomicrons . By similarity.Phosphorylation sites are present in the extracellular medium . By similarity. Sequence similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.

1 mL

600 USD