product summary
Loading...
company name :
MyBioSource
product type :
antibody
product name :
DnaK
catalog :
MBS600301
quantity :
0.05 mg
price :
460 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[4E66]
application :
enzyme immunoassay
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS600301 image 1
Western Blot Analysis using MBS600301: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.
product information
catalog number :
MBS600301
products type :
Antibody
products full name :
DnaK
products short name :
[DnaK]
products name syn :
[Anti -DnaK]
other names :
[DnaK]
clonality :
Monoclonal
isotype :
IgG1
clone :
[4E66]
host :
Mouse
reactivity :
E Coli
specificity :
Recognizes E. coli DnaK.
purity :
Affinity Purified. Purified by Protein G affinity chromatography.
form :
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
concentration :
1mg/ml
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
ELISA (EL/EIA), Western Blot (WB). Other applications not tested.
app notes :
Western Blot: 1:10,000. Detects a band at ~70kD. Optimal dilutions to be determined by researcher. Positive control: Dnak, Recombinant, E.coli
image1 heading :
Western Blot (WB)
other info1 :
Immunogen: E. coli DnaK
other info2 :
Control No.: C14121956. Revision No.: 072512
products categories :
Antibodies; Abs to Proteins
products description :
DnaK possesses an autophosphorylation activity and a weak 5'-nucleotidase activity, cleaving the 5'-phosphate groups from both ribose- and deoxyribose nucleotides. Genetic and biochemical studies have demonstrated protein-protein interactions between the DnaK protein and the lambdaO and lambdaP DNA replicating proteins. DnaK has also been shown to interact with DnaJ and GrpE heat shock proteins. In addition, DnaK has been demonstrated to preferentially bind to the unfolded form of bovine pancreatic trypsin inhibitor as opposed to the native protein. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology. Like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes, essentially as first proposed by Hightower and Pelham.
ncbi gi num :
6746608
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size1 :
0.05 mg
price1 :
460 USD
size2 :
0.2 mg
price2 :
745
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!