antibody

SNAP 25, aa183-197 (Protein 25kDa, SNAP25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, RIC4, EC9, SNAP, Super Protein, SUP)

MBS6001675

1 mL

725 USD

monoclonal

mouse

nonconjugated

9L650

ELISA, immunohistochemistry, enzyme immunoassay

Antibody

SNAP 25, aa183-197 (Protein 25kDa, SNAP25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, RIC4, EC9, SNAP, Super Protein, SUP)

SNAP 25, aa183-197

Anti -SNAP 25, aa183-197 (Synaptosomal-associated Protein 25, Synaptosomal Associated Protein 25, Synaptosomal-associated 25kD Protein, Synaptosomal-associated Protein 25kDa, SNAP25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, Resistance to Inhibitor

synaptosomal-associated protein 25 isoform a; Synaptosomal-associated protein 25; synaptosomal-associated protein 25; SUP; SNAP-25; super protein; synaptosomal-associated 25 kDa protein; synaptosomal-associated protein, 25 kDa; synaptosomal-associated protein 25; Super protein; SUP; Synaptosomal-associated 25 kDa protein

Snap25; Snap25; SNAP-25B; SNAP-25a; Snap; SNAP-25; SUP

SNP25_RAT

Monoclonal

IgG

9L650

Mouse

Recognizes the C. botulinum Toxin A cleaved form of SNAP 25, aa183-197. Has been found to bind only to C. botulinum Toxin A cleaved SNAP 25 by ELISA and cell staining techniques. Does not react with SNAP-25 (uncleaved by C.botulinum Toxin A), VAMP2 or Syntaxin 1.

Supernatant. Supernatant

Supplied as a liquid. No preservative added.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Immunohistochemistry (IHC)

Suitable for use in ELISA and Immunohistochemistry. Optimal dilutions to be determined by the researcher.

Immunogen: Synthetic peptide corresponding to aa183-197 of analogue SNAP 25.

Antibodies; Abs to Neuroscience

SNAP 25 (synaptosome-associated protein of 25kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26aas, respectively, from the C-terminus. The protein is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin/VAMP. The protein is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells.

396080316

NP_001257505.1

NM_001270576.1

P60881

23,315 Da

Acetylcholine Neurotransmitter Release Cycle Pathway (829219); GABA Synthesis, Release, Reuptake And Degradation Pathway (829220); Glutamate Neurotransmitter Release Cycle Pathway (829217); Insulin Signaling Pathway (198452); Insulin Secretion Pathway (777547); Integration Of Energy Metabolism Pathway (828783); Metabolism Pathway (828668); Neuronal System Pathway (829209); Neurotransmitter Release Cycle Pathway (829214); Norepinephrine Neurotransmitter Release Cycle Pathway (829215)

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein. It is essential for regulated exocytosis in neuronal cells. Alternative transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2012]

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described. Protein type: Vesicle. Cellular Component: voltage-gated potassium channel complex; neuron projection; cell; terminal button; intracellular organelle; trans-Golgi network; cytosol; actin cytoskeleton; lipid raft; SNARE complex; growth cone; cell soma; membrane; lamellipodium; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cell junction; vesicle; myelin sheath; endosome; filopodium. Molecular Function: SNAP receptor activity; protein domain specific binding; voltage-gated potassium channel activity; protein binding; SNARE binding; myosin binding; syntaxin-1 binding; protein N-terminus binding; calcium-dependent protein binding. Biological Process: synaptic vesicle fusion to presynaptic membrane; growth hormone secretion; exocytosis; axonogenesis; long-term memory; regulation of synaptogenesis; neurotransmitter secretion; calcium ion-dependent exocytosis of neurotransmitter; positive regulation of hormone secretion; positive regulation of insulin secretion; endosome transport; sleep; synaptic vesicle priming

1 mL

725 USD