Mouse anti-Human Troponin I, Cardiac, aa87-91 (cTnl) | MyBioSource MBS6000478 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti-Human Troponin I, Cardiac, aa87-91 (cTnl)

catalog :

MBS6000478

quantity :

1 mg

price :

755 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1.BB.146]

reactivity :

human

application :

enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS6000478

products type :

Antibody

products full name :

Mouse anti-Human Troponin I, Cardiac, aa87-91 (cTnl)

products short name :

[Troponin I, Cardiac, aa87-91]

products name syn :

[Anti -Troponin I, Cardiac, aa87-91 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)]

other names :

[troponin T, cardiac muscle isoform 4; Troponin T, cardiac muscle; troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant); troponin T type 2 (cardiac); Cardiac muscle troponin T]

other gene names :

[TNNT2; TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; TnTc; cTnT]

uniprot entry name :

TNNT2_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[1.BB.146]

host :

Mouse

reactivity :

N/A.

specificity :

Recognizes free human cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. No crossreactivity with skeletal muscle troponin I.Species Crossreactivity: bovine,porcine,goat,canine,rabbit and feline,.Not reactive with cTnl from rat, mouse or fish.

purity :

>90% Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.

form :

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

concentration :

4.7 mg/mL

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Western Blot (WB) and Immunoaffinity Purification. Other applications not tested.

app notes :

Optimal dilutions to be determined by the researcher.

other info1 :

Immunogen: Free human cardiac troponin I. Grade: Affinity Purified

other info2 :

Hybridoma: SP2/0 myeloma cells with spleen cells from Balb/c mice.

products categories :

Antibodies; Abs to Cardiac Markers

products description :

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter "I" is given due to its inhibitory character.

ncbi gi num :

48255883

ncbi acc num :

NP_001001432.1

ncbi gb acc num :

NM_001001432.2

uniprot acc num :

P45379

ncbi pathways :

Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament. Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; actin binding; tropomyosin binding. Biological Process: metabolic process; atrial cardiac muscle morphogenesis; regulation of muscle contraction; positive regulation of ATPase activity; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding. Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2

size1 :

1 mg

price1 :

755 USD

more info or order :

MyBioSource product webpage