antibody

Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1)

MBS6000269

0.1 mg

545 USD

monoclonal

mouse

nonconjugated

11n49

western blot, ELISA, immunoprecipitation, enzyme immunoassay

Antibody

Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1)

Clostridium botulinum Neurotoxin Type C1 Heavy Chain

Anti -Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1)

Monoclonal

IgG1

11n49

Mouse

Recognizes C. botulinum BoNT-C1 Heavy Chain. In direct ELISA, does not crossreact with the light chains of BoNT-A, -B,-C1,-D, -E, -F, -G, or heavy chains of BoNT-D or -G.

Affinity Purified. Purified by Protein G affinity chromatography.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

~0.5mg/ml (after reconstitution)

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1ug/ml. Immunoprecipitation: 25ug/ml

Immunogen: Recombinant protein corresponding to aa866-1291 from C. botulinum BoNT-C1 heavy chain, expressed in E. coli (P18640).

Antibodies; Abs to Infectious Disease

BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100kD C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn2+-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291aa, 150kD C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100kD C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50kD N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa866-1291, the C1 heavy chain shares less than 40aa identity with the D-type neurotoxin heavy chain.

0.1 mg

545 USD