Somatostatin Receptor Type 2 (SSTr2) | MyBioSource MBS600002 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Somatostatin Receptor Type 2 (SSTr2)

catalog :

MBS600002

quantity :

0.1 mg

price :

680 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[8B44]

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry analyis of SSTR2 in human brain. SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using MBS600002 (3ug/ml) overnight at 4 C. Tissue was stained with the Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).

image 2 :

Immunohistochemistry analyis of SSTR2 in MDA-MB-231 human cell line. SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using MBS600002 (10ug/ml) for 3 hours at RT. Cells were stained with NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (brown) and counterstained with DAPI (blue).

image 3 :

Flow Cytometry analyis of SSTR2 in MDA-MB-231 human cell line. MDA-MB-231 human breast cancer cell line was stained with MBS600002 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab )2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

product information

catalog number :

MBS600002

products type :

Antibody

products full name :

Somatostatin Receptor Type 2 (SSTr2)

products short name :

[Somatostatin Receptor Type 2]

products name syn :

[Anti -Somatostatin Receptor Type 2 (SSTr2)]

other names :

[somatostatin receptor type 2; Somatostatin receptor type 2; somatostatin receptor type 2; SS2R; SRIF-1; somatostatin receptor 2; SRIF-1]

other gene names :

[SSTR2; SSTR2; SSTR2]

uniprot entry name :

SSR2_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[8B44]

host :

Mouse

reactivity :

Human

specificity :

Recognizes human Somatostatin Receptor Type 2. Stains SSTr2 transfectants but not irrelevant transfectants.

purity :

Purified by Protein A affinity chromatography from hybridoma culture supernatant.

form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

concentration :

~0.5 mg/mL (after reconstitution).

storage stability :

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

app notes :

Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Dilution: Flow Cytometry (Intercellular staining): 2.5ug labels 10e6 cells. MDA-MB-231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml. Immersion fixed MDA-MB-231 human breast cancer cell line. Immunohistochemistry: 8-25ug/ml. Immersion fixed paraffin-embedded sections of human brain (cingulate cortex). Optimal dilutions to be determined by researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Immunogen: NReocmbinant protein corresponding to Met1-Ile369 from human Somatostatin Receptor Type2, expressed in NSO cells.

products categories :

Antibodies; Abs to Receptors

products description :

Somatostatin Receptor 2 (SSTr2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTr2 shares 84% aa identity with mouse SSTr2 within the extracellular domains. Isoform B (357aa) has an alternate C-terminal cytoplasmic region that is 12aa shorter than that of isoform A (369aa, reported as 93kD). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.

ncbi gi num :

4557859

ncbi acc num :

NP_001041.1

ncbi gb acc num :

NM_001050.2

uniprot acc num :

P30874

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); GPCRs, Other Pathway (198765); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney. [provided by RefSeq]

uniprot summary :

SSTR2: Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels. The C-terminus interacts with SHANK1 PDZ domain. Cerebrum and kidney. In lesser amounts in jejunum, colon and liver. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 17q24. Cellular Component: neuron projection; integral to plasma membrane; cytoplasm; plasma membrane. Molecular Function: neuropeptide binding; somatostatin receptor activity; protein binding; PDZ domain binding. Biological Process: peristalsis; regulation of muscle contraction; response to starvation; negative regulation of cell proliferation; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; cell-cell signaling; neuropeptide signaling pathway; forebrain development; digestion; cerebellum development; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; response to nutrient

size1 :

0.1 mg

price1 :

680 USD

more info or order :

MyBioSource product webpage