other

Zika Real Time RT-PCR Kit

MBS598109

25 Tests/Kit (Instr.

570 USD

PCR Kit

Zika Real Time RT-PCR Kit

Zika

All reagents should be stored at -20 degree C. Storage at +4 degree C is not recommended. Please refer to the pdf manual for more specific storage instructions.

Samples: Serum or plasma by using real time PCR systems.

Kit Series of Insect Vector

Intended Uses: Zika Virus (ZIKV) Real Time RT-PCR Kit is used for the detection of Zika virus in serum or plasma by using real time PCR systems. Principle of the Assay The principle of the real-time detection is based on the fluorogenic 5'nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5' end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.

Charrel RN, Leparc-Goffart I, Pas S, de Lamballerie X, Koopmans M & Reusken C. State of knowledge on Zika virus for an adequate laboratory response [Submitted]. Bull World Health Organ E-pub: 10 Feb 2016. . Clin Infect Dis. (2016) doi: 10.1093/cid/ciw210

25 Tests/Kit (Instr. I, II Recommended)

570 USD

25 Tests/Kit (Instr. III, IV Recommended)

570