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Recombinant S. typhi outer membrane protein

MBS596008

1 mg

315 USD

Antigen

Recombinant S. typhi outer membrane protein

[S. tyhi outer membrane]

[Recombinant S. tyhi OMP antigen]

>95% pure (10% SDS-PAGE, Coomassie blue stain)

Purified, Liquid

l.23 mg/ml

Upon receipt, aliquote and store at -20 degree C, avoid repeated freezing and thawing.

Lateral flow rapid test, Immunoassay

Test Data

Protein Buffer: PBS. Source: E.coli

Tag: 6 x Histidines. Conditons For Product Development: It is suggested to use 16ug/ml antigen for gold concentration (OD 1.2 to 1.4) to conjugate to fresh prepared colloid gold with wavelength 528 to 530. The conjugation pH and time is 8.5 and 8-9 hours. Buffer: 10mM Tris-HCI, ImM EDTA, 50mM arginine

S. typhi is a common pathogen causing typhoid fever, it affects more than 17 million people with nearly 600,000 deaths annually in the world; untreated typhoid fever cases result in mortality rates ranging from 12-30%. S. typhi outer membrane protein is an important immunogen to react to specific IgM and JgG from infected patient, it is also a potential target for vaccine development. This recombinant S. typhi OMP has 315 amino acids with 33 kDa molecular size, a 6 x his tag is fused at its C-terminus. In house prepared lateral flow test, S. typhi JgG and IgM can be detected when OMP with LPS conjugated together to colloid gold at pH 9 to 9.5. It also be used for other test such as ELISA to test specific S. typhi IgG and IgM.

35kDa

1 mg

315 USD

2 mg

545

5 mg

1220