product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Multiplex Human Cytokine ELISA Kit
catalog :
MBS590064
quantity :
96 Wells
price :
1075 USD
more info or order :
product information
catalog number :
MBS590064
products type :
ELISA Kit
products full name :
Multiplex Human Cytokine ELISA Kit
products short name :
[Inflammatory Cytokine]
products name syn :
[Inflammatory Cytokine (IL-1a, IL-1b, IL-6, IL-8, GM-CSF, INF-g, MCAF and TNF-a)]
specificity :
This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MUC5AC and all the analogues, therefore, cross reaction may still exist in some cases.
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 0.1 ng/mL.
products categories :
Multiplex Human Cytokine ELISA Kits; Multiplex Human Cytokine ELISA Kits
products description :
Intended Uses: This MUC5AC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MUC5AC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MUC5AC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MUC5AC antibody and an MUC5AC-HRP conjugate. The assay sample and buffer are incubated together with MUC5AC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC5AC concentration since MUC5AC from samples and MUC5AC-HRP conjugate compete for the anti-MUC5AC antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC5AC from the sample, fewer sites are left to bind MUC5AC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC5AC concentration in each sample is interpolated from this standard curve.
size1 :
96 Wells
price1 :
1075 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!