ELISA/assay

Interleukin-17A (IL-17A)

MBS590052

96 Wells

580 USD

ELISA Kit

Interleukin-17A (IL-17A)

Interleukin-17A

interleukin-17A; Interleukin-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8

IL-17A

IL17A; IL17A; IL17; CTLA8; IL-17; IL-17A

IL17_HUMAN

155

This sandwich ELISA recognises both natural and recombinant human IL-17A. This kit has been tested and exhibited no significant cross-reactivity with following human cytokines and growth factors: IL-1alpha, IL-1beta, IL-2, IL-4, IL-5, Il-6, IL-7, IL-8, IL-10, IL-11, Il-12, Il-13Ra2, FGF basic, GM-CSF, IFN-gamma, M-CSF, MCP-1(MCAF), MCP3, EGF, TNF- alpha, TNF-beta.

Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids. Assay Type: Sandwich. Sensitivity: The minimum detectable dose of IL-17A was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-17A calculated from calibrate I diluted standard curve was <5 pg/mL.

Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 6 different assays. Intended Uses: This Human Interleukin-17A ELISA Kit is to be used for the in vitro quantitative determination of human interleukin-17A (IL-17A) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH ONLY and is not to be used for diagnostic or therapeutic procedures.

ELISA Kits for Human Cytokines and Receptors

Principle of the assay: This IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for IL-17A was added and incubated. IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-17A and other components of the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-17A concentration (pg/mL). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Background: Interleukin -17A (IL-17A) is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of 35 Kilo Dalton. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL- 17D, IL-17E (IL-25) and IL-17F. All the IL-17s have similar structure with four highly conserved cysteine residues and same 3 dimensional structures, which distinguish this family from other cytokines. IL-17 is produced by a group of CD4 T help cells termed Th17 cells. In addition to previous discovered Th1 and Th2 cells, the Th17 cells consist in the third group of T help cells. IL-23, a growth and stabilization factor was the first cytokine that was found to involve with the development of Th 17 cells. Later on, other cytokines including TGF-beta, IL-6, IL-21 were also found to contribute to the procedure. The transcription factors implicated in the procedure were identified as STAT3, RORgt, and RORa. IL-17, like interferon-gamma, is a potent mediator of delayed-type reaction. IL-17 induces the secretion of many other pro-inflammatory cytokines and chemokines such as IL-6, IL-8, GM-CSF, GCSF, TGF-beta, TNF-alpha, GRO-alpha, MCP-1, and stimulates the expression of NFkappaB, mitogen activated protein kinase, and prostaglandings from many cell types. By stimulating cytokine production and recruiting monocytes neutrophils to site of inflammation, the cytokine exerts its effect in response to tissue damage and invasion. Excessive expression of IL-17 was found to associate with autoimmune diseases and chronic inflammatory diseases such as multiple sclerosis, rheumatoid arthritis, psoriasis, inflammatory bowel diseases, tissue allograft rejection, allergic asthma and infectious diseases at the mucosal tissue.

4504651

NP_002181.1

NM_002190.2

17,504 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968); IL23-mediated Signaling Events Pathway (138000); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); TCR Signaling Pathway (198862)

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: extracellular space; external side of plasma membrane. Molecular Function: cytokine activity. Biological Process: cell death; cell-cell signaling; positive regulation of interleukin-23 production; apoptosis; positive regulation of osteoclast differentiation; protein amino acid glycosylation; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

96 Wells

580 USD