ELISA/assay

Serum Amyloid A (SAA)

MBS590049

96 Wells

640 USD

ELISA Kit

Serum Amyloid A (SAA)

Serum Amyloid A

serum amyloid A protein; serum amyloid A protein

SAA

SAA1; SAA

129

This ELISA recognises both natural and recombinant human SAA. This kit exhibits no detectable cross-reactivity with human; IL-8, IL-1beta, MCAF, FGF, TGF-beta, EGF, GMCSF, M-CSF, MCP-3, TNF-alpha, RANTES, and EPO.

Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids. Assay Type: Sandwich. Sensitivity: The minimum detectable dose of SAA was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human SAA using a standard curve generated with Calibrator Diluent I is 1.1 ng/mL and using Calibrator Diluent II is 0.6 ng/mL.

Intra-assay Precision: To detemine within-run precision, three different samples of known concentration were assayed by using 20 replicates in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 16-20 different assays. Intended Uses: This Human SAA ELISA Kit is to be used for the in vitro quantitative determination of human serum amyloid A (SAA) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORTORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

ELISA Kits for Human Inflammatory and Autoimmune Conditions

Principle of the assay: This SAA enzyme-linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for SAA. Standards or samples are then added to the appropriate microtiter plate wells and incubated. SAA if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound SAA and other components of sample. In order to quantitate the amount of SAA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for SAA is added to each well to "sandwich" the SAA immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate solution are allowed to react over a short incubation period. Only those wells that contain SAA and enzyme-substrate reaction will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of SAA in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus SAA (ng/mL). The concentration of SAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

93352563

NP_001035288.1

NM_001040198.1

96 Wells

640 USD