ELISA/assay

Alpha Fetoprotein (AFP)

MBS590043

96 Wells

485 USD

ELISA Kit

Alpha Fetoprotein (AFP)

Alpha Fetoprotein

alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

AFP

AFP; AFP; AFPD; FETA; HPAFP

FETA_HUMAN

609

This kit exhibits no detectable cross-reactivity with CEA, PSA, HCG, CA125, CA153, and human serum albumin.

Samples: Human blood samples and Cell Culture Supernatant. Assay Type: Sandwich. Sensitivity: The minimum detectable dose of AFP was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human AFP using a standard curve generated is 2.0 ng/mL.

Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 15 replicates in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 15 different assays. Intended Uses: This AFP ELISA Kit is to be used for the in vitro quantitative determination of alpha-fetoprotein in human blood samples and cell culture supernatant. This kit is intended FOR LABORTORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

ELISA Kits for Human Tumor Markers

Principle of the assay: This AFP enzyme-linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AFP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. AFP if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound AFP and other components of sample. In order to quantitate the amount of AFP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for AFP is added to each well to "sandwich" the AFP immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AFP and enzyme-substrate reaction will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of AFP in the samples, this kit includes a set standard. This allows the operator to produce a standard curve of Optical Density (O.D) versus AFP (ng/mL). The concentration of AFP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Background: Alpha-fetoprotein (AFP) is a glycoprotein with a molecular mass of 65,000 Daltons. It is normally produced in large amounts by liver, the visceral endoderm of foetus. Alpha-fetoprotein level increases progressively and reaches a peak at the 30th week in the maternal serum. Thereafter, it decreases gradually and reduces to trace amount with a couple of years after birth. Human AFP gene is located at the chromosomal location 4q25. The expression of AFP is regulated by a large promoter P1 and three distant enhancers. AFP expression abnormality is a relatively common genetic disorder that affects intellectual development and causes other health problems. Increased AFP expression is observed in adults that developed metastasis of liver and other organs. AFP is thought to be a foetal form of serum albumin and exists in monomeric, dimeric and trimeric forms. It binds to copper, nickel, fatty acids and bilirubin. The exact function of human AFP is still under investigation. Several hypotheses have been proposed for the physiological function: regulation of cell growth, sexual differentiation, transportation of metals and other substances, interaction with cytoplasmic chaperone proteins, and protecting foetus against the maternal immunity. To cover the very large range of concentration associated with the great variety of processes, the current immunoassay is a simple tool for the detection of AFP in the range 0 - 400ng/ml.

178236

AAB58754.1

68,678 Da

Direct P53 Effectors Pathway (137939); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; cytoplasm. Molecular Function: metal ion binding. Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle. Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

96 Wells

485 USD