ELISA/assay

Macrophage Colony Stimulating Factor (M-CSF)

MBS590023

96 Wells

580 USD

ELISA Kit

Macrophage Colony Stimulating Factor (M-CSF)

Macrophage Colony Stimulating Factor

macrophage colony-stimulating factor 1 isoform b; Macrophage colony-stimulating factor 1; macrophage colony-stimulating factor 1; lanimostim; colony stimulating factor 1 (macrophage); Lanimostim

M-CSF

CSF1; CSF1; MCSF; CSF-1

CSF1_HUMAN

438

This sandwich ELISA can detect both natural and recombinant human M-CSF. This kit exhibits no significant cross-reactivity with human IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-7, IL-8, IL-15, IL-16, TNF-alpha, TGF-beta, and TNF-beta.

Intended Uses: This Human M-CSF ELISA Kit is to be used for the in vitro quantitative determination of human Macrophage Colony Stimulating Factor (M-CSF) concentrations in serum, plasma and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

Sensitivity: The minimum detectable quantities of human M-CSF as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 15 pg/mL and 37pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities. Intra-assay Precision: To determine within-run precision, 3 different sera were assayed by using 10 replicates in 1 assay. Inter-assay Precision: To determine between-run precision, 3 different sera were assayed by using replicates on 20 different assays.

ELISA Kits for Human Cytokines and Receptors

Principle of the assay: This M-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to M-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-CSF and incubated. If present, M-CSF will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound MCSF and other components of the sample. In order to quantitatively determine the amount of M-CSF present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain M-CSF, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of M-CSF in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus M-CSF concentration (pg/mL). The concentration of M-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

27262663

NP_757349.1

NM_172210.2

29,215 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!Integrins In Angiogenesis Pathway 137999!!Osteoclast Differentiation Pathway 193147!!Osteoclast Differentiation Pathway 193096!!PI3K-Akt Signaling Pathway 692234!!PI3K-Akt Signaling Pathway 692979

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

96 Wells

580 USD