ELISA/assay

Interleukin-6 Soluble Receptor (IL-6 sR)

MBS590012

96 Wells

580 USD

ELISA Kit

Interleukin-6 Soluble Receptor (IL-6 sR)

Interleukin-6 Soluble Receptor

interleukin-6 receptor subunit beta isoform 1; Interleukin-6 receptor subunit beta; interleukin-6 receptor subunit beta; CD130 antigen; IL-6 receptor subunit beta; IL-6R subunit beta; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130, oncostatin M receptor; interleukin receptor beta chain; membrane glycoprotein 130; membrane glycoprotein gp130; oncostatin-M receptor subunit alpha; interleukin 6 signal transducer; CDw130; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD_antigen: CD130

IL-6 sR

IL6ST; IL6ST; CD130; GP130; CDW130; IL-6RB

IL6RB_HUMAN

918

This sandwich ELISA recognises both natural and recombinant human IL-6 sR. This kit exhibits no significant cross-reactivity with factors related to or associated with IL-6 sR such as rhIL-6, rhsgp130 or rmIL-6. No significant cross-reactivity was observed with recombinant human: IL-1alpha, IL-1beta, IL-1ra, IL-2 IL-3, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, beta-ECGR, EGF, EPO, FGF acidic, FGF basic, FGF-4, G-CSF, GM-CSF, sgp 130, GRO alpha, IFN-gamma, IGF-I IGF-II, LIF, M-CSF, MCP-1, MIP-1alpha, MIP-1beta, OSN, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, SCF, SLPI, TGF- alpha, TGF-beta, TNF- alpha, TNF- beta, sTNF RI, sTNF RII.

Intended Uses: This Human Interleukin-6 soluble receptor ELISA Kit is to be used for the in vitro quantitative determination of human interleukin-6 soluble receptor (IL-6sR) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH ONLY and is not to be used for diagnostic or therapeutic procedures.

Sensitivity: The minimum detectable dose of IL-6sR was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-6sR using a standard curve generated with Calibrator Diluent I is <7 pg/mL and using Calibrator Diluent II is <7pg/mL. Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.

ELISA Kits for Human Cytokines and Receptors

Principle of the assay: This IL-6 sR enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-6sR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6sR and incubated. IL-6sR if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-6sR and other components of the sample. In order to quantitatively determine the amount of IL-6sR present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-6sR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-6sR in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-6sR concentration (pg/mL). The concentration of IL-6sR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

28610147

NP_002175.2

NM_002184.3

96,276 Da

ARMS-mediated Activation Pathway 106466!!Activated TLR4 Signalling Pathway 106400!!Adipogenesis Pathway 198832!!Axon Guidance Pathway 105688!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Developmental Biology Pathway 477129

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

96 Wells

580 USD