ELISA/assay

Interleukin-2 Soluble Receptor alpha (IL-2 sRa)

MBS590008

96 Wells

580 USD

ELISA Kit

Interleukin-2 Soluble Receptor alpha (IL-2 sRa)

Interleukin-2

interleukin-2 receptor subunit alpha; Interleukin-2 receptor subunit alpha; interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; TAC antigen; interleukin 2 receptor, alpha; TAC antigen; p55; CD_antigen: CD25

IL-2 sRa

IL2RA; IL2RA; p55; CD25; IL2R; TCGFR; IDDM10

IL2RA_HUMAN

272

This sandwich ELISA can detect both natural and recombinant human IL-2 sRalpha. This kit exhibits no significant cross-reactivity with factors related to or associated with IL-2 sRalpha such as rhIL-2, rhIL-2 sRalpha. No significant cross-reactivity was observed with recombinant human: IL-1alpha, IL-1beta, IL-1 sRI, IL-1 sRII, IL-1ra, IL-3, IL-3 sR alpha, IL-5, IL-5 sR alpha, IL-5 sRb, IL-6, IL-6 sR, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13 ANG, AR, CNTF, beta-ECGR, EGF, EPO, FGF acidic, FGF basic, FGF-4, FGF-5, FGF-6, FGF-7, GCSF, GM-CSF, sgp 130, GRO alpha, GRObeta, GROgamma, HB-EGF, HGF, INF-gamma, IGF-I IGF-II, LAP (TGF-beta1), LIF, M-CSF, MCP-1, MIP-1alpha, MIP-1beta, beta-NGF, OSN, PD-ECGF, PDGFAA, PDGF-AB, PDGF-BB, PTN, RANTES, SCF, SLPI, TGF- alpha, TGF-beta, TNF- alpha, TNF- beta, sTNF RI, sTNF RII, VEGF.

Intended Uses: This Human Interleukin-2 soluble receptor alpha ELISA Kit is to be used for the in vitro quantitative determination of human interleukin-2 soluble receptor alpha (IL-2 sRalpha) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not to be used in diagnostic or therapeutic procedures.

Sensitivity: The minimum detectable quantity of human IL-2 sRalpha as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II is 10 pg/mL. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities. Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.

ELISA Kits for Human Cytokines and Receptors

Principle of the assay: This IL-2 sRalpha enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-2 sRalpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-2 sRalpha and incubated. IL-2 sRalpha, if present, will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL- 2 sRalpha and other components of the sample. In order to quantitatively determine the amount of IL-2sRalpha present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-2 sRalpha, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. In order to measure the concentration of IL-2 sRalpha in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 sRalpha concentration (pg/mL). The concentration of IL-2 sRalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4557667

NP_000408.1

NM_000417.2

30,819 Da

Arf6 Trafficking Events Pathway 137954!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181!!G Beta:gamma Signalling Through PI3Kgamma Pathway 119540

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

96 Wells

580 USD