ELISA/assay

Interferon-Gamma (IFN-gamma), human

MBS590004

96 Wells

580 USD

ELISA Kit

Interferon-Gamma (IFN-gamma), human

Interferon-Gamma

Interferon-Gamma (IFN-gamma); Interferon-Gamma (IFN-g)

interferon-gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; interferon, gamma; Immune interferon

IFN-gamma

IFNG; IFNG; IFG; IFI

IFNG_HUMAN

166

This sandwich ELISA recognizes both natural and recombinant human IFN-gamma. This kit exhibits no detectable cross-reactivity with human; SAA, EGF, IL-6, IL-8, IL-16, M-CSF, GM-CSF, TGF-beta1, RANTES, FGF, MIP-1alpha, CRP, EPO.

Intended Uses: This Human IFN-gamma ELISA kit is to be used for the in vitro quantitative determination of concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

Sensitivity: The minimum detectable dose of IFN-gamma was determined by adding two standard deviations to the mean optical density value of the 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 13.1 pg/mL and using Calibrator Diluent II is 7.5 pg/mL. Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay. Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.

ELISA Kits for Human Cytokines and Receptors

Principle of the assay: This IFN-gamma enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for IFN-gamma. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IFN-gamma, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IFN-gamma and other components of sample. In order to quantitatively determine the amount of IFN-gamma present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IFN-gamma is added to each well to "sandwich" the IFN-gamma immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-gamma and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm.

184639

AAB59534.1

19,348 Da

ATF-2 Transcription Factor Network Pathway 138006!!African Trypanosomiasis Pathway 194384!!African Trypanosomiasis Pathway 194323!!Allograft Rejection Pathway 920963!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

96 Wells

580 USD