antibody

Iron Regulatory Protein 1 (IRP1) Rabbit Polyclonal Antibody

MBS588254

0.05 mL

435 USD

polyclonal

rabbit

nonconjugated

human, mouse

Antibody

Iron Regulatory Protein 1 (IRP1) Rabbit Polyclonal Antibody

[Iron Regulatory Protein 1 (IRP1)]

[Iron-responsive element-binding proteins; IRE-BP; IRBR IRP; IFR]

[iron regulatory protein 1; Cytoplasmic aconitate hydratase; cytoplasmic aconitate hydratase; aconitase 1; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1]

[IRP1]

[ACO1; ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1; IREB1; Aconitase; IRP1; IRE-BP 1]

Polyclonal

Rabbit

Human, Mouse

790

The antibody recognizes the full length IRP1 protein

Lyophilized serum

Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C). For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

If reconstituted in 50 muL: WB: 1:2000.

Immunogen: Full length protein

Preservative: None

The iron regulatory proteins IRP1 and IRP2 are involved in posttranscriptional regulation of cellular iron metabolism by binding to mRNA iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. IRP-1 is a bifunctional soluble protein that functions as an IRE-binding protein or as the cytoplasmic isoform of aconitase.

1. Fillebeen C-A phosphomimetic mutation at Ser-138 renders iron regulatory protein 1 sensitive to iron-dependent degradation.

9802308

AAF99681.1

98,399 Da

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Antibiotics Pathway (1144997); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811)

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

0.05 mL

435 USD