antibody

Rat anti-CRB3 (14F9)

MBS588104

0.1 mg

555 USD

monoclonal

rat

nonconjugated

14F9

western blot, ELISA, immunohistochemistry, immunocytochemistry

Antibody

Rat anti-CRB3 (14F9)

CRB3

CRB3; Protein crumbs homolog 3; protein crumbs homolog 3; crumbs protein homolog 3; crumbs family member 3

CRB3

CRB3; CRB3

CRUM3_HUMAN

Monoclonal

IgG2c

14F9

Rat

The antibody recognizes the CRB3 protein.

123

Specific for: human and mouse; other species not tested.

100 ug of lyophilized, purified antibody. Reconstitute in 100 ul of distilled H2O for a 1 ug/ul solution. It contains no additives.

Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at -20 degree C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Stability: Minimum 1 year from reception date.

ELISA (E), Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

If reconstituted in 100 ul: WB 1:1000, IHC 1:100, IF 1:100. Optimal dilution has to be determined by the user.

Limitations: This product is to be used for research purposes only.

CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.

37182490

AAQ89047.1

Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Tight Junction Pathway (83071); Tight Junction Pathway (482); Tight Junction Interactions Pathway (119535)

This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

CRB3: Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: tight junction; protein complex; apical plasma membrane; plasma membrane; integral to membrane; cell junction. Molecular Function: protein domain specific binding; SH3 domain binding. Biological Process: intercellular junction assembly and maintenance

0.1 mg

555 USD