antibody

CD163, Human, mAb RM3/1

MBS584200

0.1 mg

555 USD

monoclonal

human

nonconjugated

human, rat

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

CD163, Human, mAb RM3/1

CD163

Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

CD163; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen; CD163 molecule; Hemoglobin scavenger receptor; CD_antigen: CD163Cleaved into the following chain:Soluble CD163; sCD163

CD163

CD163; CD163; M130; MM130; M130; sCD163

C163A_HUMAN

Monoclonal

Mouse IgG1

Human

Cross Reactant:. Monkey = Yes. Rat = No. Pig = No. Guinea pig = No

1116

1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

F, Flow Cytometry (FC/FACS), FS, Immunoflurescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Application Notes: FC: Permeabilization as well as no permeabilization is used. Block cells with 1% BSA for 30';at 4 degree ;C (Ref 5). Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. CD163 is sensitive to ionic detergents and to chloroform/methanol treatment.

Immunogen: Human monocytes (epitope recognized SRCR domain 9)

Positive Control: CHO-cells transfected with CD163. Negative Control: Muscle cells. Precautions: For research only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. MBS is not responsible for any paten infringements that might result from the use or derivation of this product.

Product Description: The monoclonal antibody RM3/1 recognizes CD163, a 130 kDa type I membrane glycoprotein, which is expressed exclusively in the monocyte/macrophage system. CD163 is a member of the cysteine-rich scavenger receptor superfamily. CD163 is an acute phase regulated receptor for the hemoglobin-haptoglobin complex. Another important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels are found on tissue macrophages whereas bone marrow-derived cells are CD163 negative. CD163 positive cells include skin histiocytes, gut, Kupffer cells, and macrophages in spleen, thymus placenta and inflamed or tumorous tissues. The protein expression is markedly induced by glucocorticoids, IL-6 and IL-10 while down-regulated by cyclosporin A and by phorbol esters. CD163 can be cleaved to release to soluble form (sCD163). PMA can induce the shedding of sCD163. Intravenous lipopolysaccharide (LPS) produces a rapid rise of sCD163 in plasma of patient as it induces metalloproteinase-mediated shedding from monocytes surface. sCD163 in plasma is a parameter in diseases affecting macrophage function and monocyte/macrophage load in the body. The concentration of sCD163 is probably reflecting the number of macrophages of the 'alternative macrophage activation' phenotype with a high CD163 expression playing a major role in dampening the inflammatory response and scavenging components of damaged cells. As such sCD163 can be important as a disease marker in inflammatory conditions e.g. infection, autoimmune disease, transplantation, atherosclerosis and cancer. The monoclonal antibody RM3/1 can be used for macrophage phenotyping. The RM3/1 antigen expression is restricted to human monocytes and macrophages that evolve in the late phase of inflammation, predominantly macrophages of healing tissue. For macrophages in the synovialis of patients with rheumatoid arthritis; in alveolar macrophages and in Kupffer cells a double staining can be observed with the monoclonal antibody 25F9 (HM2158) recognizing- mature macrophages, which is not the case in other tissues.

1. Zwadlo, G et al; A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Expl Cell Biol 1987, 55: 295. 2. H¶gger, P et al; Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163). J Immunol 1998, 161: 1883. 3. Droste, A et al; Shedding of CD163, a novel regulatory mechanism for a member of the scavenger receptor cysteine-rich family. Biochem Biophys Res Commun 1999, 256: 110. 4. Frings, W et al; Only the soluble form of the scavenger receptor CD163 acts inhibitory on phorbol ester-activated T-lymphocytes, whereas membrane-bound protein has no effect. FEBS Lett 2002, 526: 93. 5. Komohara, Y et al; AM-3K, an anti-macrophage antibody, recognizes CD163, a molecule associated with an anti-inflammatory macrophage phenotype. J Histochem Cytochem 2006, 54: 763

5107945

CAB45233.1

Q86VB7

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: integral to plasma membrane; plasma membrane; extracellular region. Molecular Function: protein binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; acute-phase response

0.1 mg

555 USD