antibody

FHL2, Human, mAb F4B2-B11

MBS584089

0.1 mg

720 USD

monoclonal

mouse

nonconjugated

[F4B2-B11]

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Antibody

FHL2, Human, mAb F4B2-B11

[FHL2]

[four and a half LIM domains protein 2; Four and a half LIM domains protein 2; four and a half LIM domains protein 2; LIM domain protein DRAL; aging-associated gene 11; skeletal muscle LIM-protein 3; down-regulated in rhabdomyosarcoma LIM protein; four and a half LIM domains 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM-3]

[FHL2]

[FHL2; FHL2; DRAL; AAG11; FHL-2; SLIM3; SLIM-3; DRAL; SLIM3; FHL-2; SLIM-3]

FHL2_HUMAN

Monoclonal

IgG1

[F4B2-B11]

Mouse

Protein G

0.2 um filtered in PBS+0.1%BSA+0.02%NaN3

0.1 mg/ml

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB)

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. IF: MBS584089 is useful for immunofluorescence on paraformaldehyde fixed cells.

The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.

1. Samson, T et al; The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor. J Biol Chem 2004, 279: 28641 2. Martin, B et al; The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts. J Cell Biol 2002, 159: 113

87159836

NP_001034581.1

NM_001039492.2

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); PPARA Activates Gene Expression Pathway (530749); RANKL/RANK Signaling Pathway (672447); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544)

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Aug 2011]

FHL2: May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 2q12.2. Cellular Component: nucleoplasm; focal adhesion; M band; Z disc; nucleus; actin cytoskeleton. Molecular Function: identical protein binding; protein binding; androgen receptor binding; zinc ion binding; transcription coactivator activity; transcription factor binding. Biological Process: osteoblast differentiation; atrial cardiac muscle cell development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; ventricular cardiac muscle cell development; response to hormone stimulus; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis

0.1 mg

720 USD