antibody

Nectin-3, Mouse, mAb 103-A1, FITC

MBS584065

0.1 mg

675 USD

monoclonal

rat

FITC

human, rat

immunocytochemistry, flow cytometry, FACS

Antibody

Nectin-3, Mouse, mAb 103-A1, FITC

[Nectin-3]

[PPR3, poliovirus receptor-related protein 3, PRR3, PVRR3, CD113]

[nectin-3 isoform 1; Nectin-3; nectin-3; nectin 3; poliovirus receptor-related protein 3; poliovirus receptor-related 3; CDw113; Poliovirus receptor-related protein 3; CD_antigen: CD113]

[PVRL3; PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3; PRR3]

PVRL3_HUMAN

Monoclonal

IgG2a

Rat

Cross reactant : Human nectin-3 . Reactivity: No. Cross reactant : Rat nectin-3 . Reactivity: No

549

1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunofluorescence (IF) 1-7 .

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. IF: Fixed in 2% paraformaldehyde in PBS optionally followed by methanol/aceton fixation . FC: 1 ug per 1 x10 6 cells. FS: injection of 1 ug into lumen of seminiferous tubules. IA: Indirect ELISA on 0.5 ug/mL mouse nectin-3 coated per well. Positive signal with 2 ug/mL antibody. IF: Cells are fixed in 2% paraformaldehyde in PBS, optionally followed by methanol/aceton fixation.

Immunogen: Extracellular domain (50-400 aa)of mouse Nectin-3 fused to IgG Fc. Aliases: Poliovirus receptor-related protein 3, Nectin cell adhesion molecule 3, CD113

Positive Control: L-cells (A cultured line of C3H mouse fibroblasts). Negative Control: Cell/tissues derived from nectin-3 knockout mouse.

Product Description: The monoclonal antibody 103-A1 recognizes mouse nectin-3. Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3alpha, -3beta and -3g of which nectin-3alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

1. Satoh-Horikawa, K et al; Nectin-3 a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities. J Biol Chem 2000, 275: 10291 2. Mizoguchi, A et al; Nectin: an adhesion molecule involved in formation of synapses. J Cell Biol, 2002, 156: 555. 3. Mueller, S et al; Loss of nectin-2 at Sertoli-spermatid junctions leads to male infertility and correlates with severe spermatozoan head and midpiece malformation, impaired binding to the zona pellucida, and oocyte penetration. Biol Reprod 2003, 69: 1330. 4. Guttman, J et al; Evidence that tubulobulbar complexes in the seminiferous epithelium are involved with internalization of adhesion junctions. Biol of Reprod 2004, 71: 548. 5. Inagaki, M et al; Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Development 2005, 132: 1525. 6. Inagaki, M et al; Role of cell adhesion molecule nectin-3 in spermatid development. Genes Cells,2006, 11: 1125. 7. Toyama, Y et al; Disruption of ectoplasmic specilizations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies. Asain J Androl 2008, 10: 577

11386199

NP_056295.1

NM_015480.2

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Nectin Adhesion Pathway (138054); Nectin/Necl Trans Heterodimerization Pathway (119534)

This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

PVRL3: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 3q13. Cellular Component: apical junction complex; postsynaptic membrane; cell-cell adherens junction; integral to membrane; plasma membrane. Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding. Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; fertilization; lens morphogenesis in camera-type eye; homophilic cell adhesion; retina morphogenesis in camera-type eye

0.1 mg

675 USD