Elafin, Human, mAb TRAB2O | MyBioSource MBS584017 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Elafin, Human, mAb TRAB2O

catalog :

MBS584017

quantity :

0.1 mg

price :

665 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS584017

products type :

Antibody

products full name :

Elafin, Human, mAb TRAB2O

products short name :

[Elafin]

other names :

[elafin preproprotein; Elafin; elafin; PI-3; trappin-2; pre-elafin; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP); peptidase inhibitor 3, skin-derived; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14]

other gene names :

[PI3; PI3; ESI; WAP3; SKALP; WFDC14; cementoin; WAP3; WFDC14; ESI; PI-3; SKALP]

uniprot entry name :

ELAF_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgG1

host :

Mouse

reactivity :

Human

sequence length :

117

form :

1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

storage stability :

Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

tested application :

Immunoassay (IA), P, Western Blot (WB)

app notes :

Application Use: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use non-reducing conditions.

products description :

Product Description: Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria. TRAB2O recognises all known Trappins via the GQDPVK epitope. TRAB2O shows strong reaction with human native full-length Elafin/SKALP and a weaker reaction with fully processed Elafin/SKALP (C-terminal 57 aa). For detection of fully processed Elafin/SKALP (C-terminal 57 aa) we advise to use HM2063. The antibody is cross reactive with Trappin family members of other species with GQDPVK epitope.

products references :

1. Vandermeeren, M et al; Development and application of monoclonal antibodies against SKALP/elafin and other trappin family members. Arch Dermatol Res 2001, 293: 343 . 2. Alkemade, J et al; Demonstration of skin-derived antileukoproteinase (SKALP) and its target enzyme human leukocyte elastase in squamous carcinoma. J Pathol 1994, 174: 121 . 3. Bergen van, H et al; Expression of SKALP/elafin during wound healing in human skin. Arch Dermatol Res 1996, 288: 458 . 4. Pfundt, R et al; Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia. J Clin Invest 1996, 98: 1389 . 5. Schalkwijk, J et al; Localization of SKALP/elafin. J Invest Dermatol 1993, 100: 390 . 6. Schalkwijk, J et al; The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a c-terminal four-disulphide core. Biochem J 1999, 340: 569 . 7. Wiedow, O et al; Elafin: an elastase-specific inhibitor of human skin. J Biol Chem 1990, 265: 14791 . 8. Huh, WK et al; Dynamic alteration of human beta-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin. J Mol Med 2002, 80: 678

ncbi gi num :

4505787

ncbi acc num :

NP_002629.1

ncbi gb acc num :

NM_002638.3

ncbi summary :

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2008]

uniprot summary :

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Protein type: Secreted; Secreted, signal peptide; Inhibitor; Extracellular matrix. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: proteinaceous extracellular matrix. Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity. Biological Process: copulation

size1 :

0.1 mg

price1 :

665 USD

more info or order :

MyBioSource product webpage