ELISA/assay

25(OH) Vitamin D ELISA Kit

MBS580159

96-Strip-Wells

440 USD

ELISA Kit

25(OH) Vitamin D ELISA Kit

[25(OH) Vitamin D]

[25(OH) Vitamin D ELISA]

Human

Product should be stored at 2-8 degree C. Product is stable for 24 months from the date of manufacturing.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Body Fluids, Tissue Homogenate Or Cell Culture Supernates. Assay Type: Sandwich. Detection Range: 156 pg/ml-10,000 pg/ml. Sensitivity: <5 pg/ml

Bone Metabolism Markers

Intended Uses: Th MMP-9 ELISA has been designed for the precise measurement of MMP-9 in human serum, plasma, body fluids, tissue homogenate or cell culture supernates. For research use only, not for use in diagnostic procedures. Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-well plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing with wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiterplate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a member of the matrix metalloproteinase (MMP) family. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis, intracerebral hemorrhage, and metastasis. MMP-9 is produced by normal alveolar macrophages and granulocytes. Sundstrom et al. (2004) suggested that plasma MMP9 level may be a marker for cardiac extracellular matrix degradation, a process involved in left ventricular remodeling.

1. Holick, MF. Vitamin D Status: Measurement, Interpretation and Clinical Application. Ann Epidemoil. 2009, 19(2):73 - 78 2. Morris H. A. Vitamin D: A Hormone for All Seasons-How Much is enough? Clin. Biochem. Rev., 2005, 26, 21-32. 3. Bikle D. D. Vitamin D and the skin. J. Bone Miner. Metab., 2010, 28, 117-30. 4. Zerwekh J. E. Blood biomarkers of vitamin D status. Am. J. Clin. Nutr., 2008, 87, 1087S-91S. 5. Moyad M. A. Vitamin D: a rapid review. Dermatol Nurs., 2009, 21, 25-30.

96-Strip-Wells

440 USD