product summary
request information :
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human ADRENOCORTICOTROPIC HORMONE (ACTH) "Ultra Sensitive" lumELISA Kit
catalog :
MBS580003
quantity :
1 x96 Wells
price :
710 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS580003
products type :
ELISA Kit
products full name :
Human ADRENOCORTICOTROPIC HORMONE (ACTH) "Ultra Sensitive" lumELISA Kit
products short name :
Adrenocorticotropic Hormone (ACTH)
products name syn :
ACTH Ultra Sensitive lumELISA; Mouse/Rat ACTH Ultra Sensitive lumELISA; ACTH Ultra Sensitive lumELISA Kit
other names :
ACTH; N/A
products gene name :
ACTH
storage stability :
Shelf Life: 18 Months
other info1 :
Method: Direct ELISA. Principle: Direct ELISA. Control Standards: 0,7,18,70,215,515 pg/ml. Sensitivity: Less than 1 pg/ml
other info2 :
Run Time: 270 min. Wavelength: 450 nm
products categories :
Endocrine Markers; Steroids
products description :
Intended Use: The CBIACTH Chemiluninescence ELISA (lumELISA) is an ultra sensitive method (Less than 1 pg/mL) intended for the quantitative determination of ACTH (Adrenocorticotropic Hormone) in Mouse/Rat plasma. Summary and Explaination: Adrenocorticotropic Hormone (ACTH) is a 39-amino acid peptide hormone (MW=4500) secreted mainly by the anterior pituitary gland. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH). CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation. Principle of the test: The CBIACTH Immunoassay is a two-site lumELISA (Chemiluninescence Enzyme-Linked ImmunoSorbent Assay) for the measurement of the biologically active 39 amino acid chain of ACTH. A goat polyclonal antibody to ACTH, purified by affinity chromatography, and a mouse monoclonal antibody to ACTH are specific for well-defined regions on the ACTH molecule. One antibody is prepared to bind only the C-terminal ACTH 34-39 and this antibody is biotinylated. The other antibody is prepared to bind only the mid-region and N-terminal ACTH 1-24 and this antibody is labeled with horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin-coated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components. Upon the addition of the luminol substrate, the enzyme activity in the enzyme-bound fraction is directly proportional to the concentration of the ACTH in the sample. A standard curve is prepared relating light unit (RLU) to the concentration of the ACTH. Concentrations of ACTH present in the controls and samples are determined directly from this curve.
ncbi gi num :
490074
ncbi pathways :
Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149
size :
1 x96 Wells
price :
710 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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