catalog number :
MBS573699
products full name :
Rabbit anti Bovine a-L-Fucosidase
products short name :
[a-L-Fucosidase]
other names :
[a-L-fucosidase]
reactivity :
Cross Reactivity: Cross-reactivities against enzymes of other sources may occur but have not been determined. Species Reactivity: Cattle
specificity :
Affinity purified antibodies of polyclonal Rabbit antiSerum to alpha-L-fucosidase from Bovine kidney.
storage stability :
The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or
below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles,
divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient
temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline
(PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not
refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by
centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted
at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
tested application :
Immunoprecipitation (IP)
app notes :
Directions for use: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between1/1 ,000 and 1/6,000.
other info1 :
Product Type: Primary Antibodies. Source Note: Source alpha -L-Fucosidase is isolated and purified from bovine kidney. Freund’s complete adjuvant is used in the first step of the immunization procedure. Physicochemical characteristics: PAb concentration 1 mg/0.5ml. No foreign proteins added. Product: Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2),stabilized with dextran. No preservative added, as it may interfere with the antibody activity. No foreign protein added.
other info2 :
Handling: The Iyophilised PAb fraction is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the sarne day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Packing: Vial with 1 mg PAb fraction, Iyophilised from 0.5 ml phosphate buffered saline. Caution: This product should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only.
products categories :
Enzyme, Metabolism, Veterinary
products description :
Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques:
immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration,
ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating
antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical
techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own
choice.
ncbi acc num :
BAB85519.1