Swine anti Human C3c, conjugated with TRITC | MyBioSource MBS573386 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Swine anti Human C3c, conjugated with TRITC

catalog :

MBS573386

quantity :

1 mL

price :

340 USD

clonality :

polyclonal

host :

pig

conjugate :

TRITC

reactivity :

human, zebrafish

application :

ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS573386

products type :

Antibody

products full name :

Swine anti Human C3c, conjugated with TRITC

products short name :

[C3c]

other names :

[complement component c3c; complement component c3c; complement component C3-2; complement component c3c]

other gene names :

[c3c; C3-3; c3-2; unp301; zgc:103610]

clonality :

Polyclonal

host :

Swine

reactivity :

Human

specificity :

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Swine antiSerum to C3c fragment of Human complement factor C3.

form :

Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phospate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

storage stability :

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

tested application :

ELISA (EIA), Immunohistochemistry/Fronzen (IHC/IHC-F), (In)direct Immunofluorescence (IF), Immunocytochemistry (ICC).

other info1 :

Conjugation: TRITC. Host Note: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure . Product: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

other info2 :

Reactivity Note: The antiSerum does not cross-react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

products categories :

Complement, Coagulation, Haematology

products description :

In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma. The fluorescent immunoconjugate to human C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

ncbi gi num :

82524318

ncbi acc num :

NP_001032313.1

ncbi gb acc num :

NM_001037236.1

uniprot acc num :

P01024

ncbi pathways :

Herpes Simplex Infection Pathway (377867); Herpes Simplex Infection Pathway (377865); Phagosome Pathway (153899); Phagosome Pathway (153859)

size1 :

1 mL

price1 :

340 USD

more info or order :

MyBioSource product webpage