Rabbit anti Saccharomyces cerevisiae Triosephosphate isomerase | MyBioSource MBS573341 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti Saccharomyces cerevisiae Triosephosphate isomerase

catalog :

MBS573341

quantity :

10 mg

price :

275 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, dot blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS573341

products type :

Antibody

products full name :

Rabbit anti Saccharomyces cerevisiae Triosephosphate isomerase

products short name :

[Triosephosphate isomerase]

other names :

[triosephosphate isomerase; Triosephosphate isomerase, chloroplastic; triosephosphate isomerase]

other gene names :

[TIM; TIM; F26H11.7; F26H11_7; PDTPI; PLASTID ISOFORM TRIOSE PHOSPHATE ISOMERASE; TIM; triosephosphate isomerase; TRIOSEPHOSPHATE ISOMERASE; TIM]

uniprot entry name :

TPIC_ARATH

clonality :

Polyclonal

host :

Rabbit

reactivity :

Saccharomyces cerevisiae

specificity :

IgG fraction of polyclonal Rabbit antiSerum to triosephosphate isomerase from baker's yeast.

storage stability :

The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots,frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4 degree C at least 10 years. Reconstituted at or below -20 degree C -3-5 years. Reconstituted at +4 degree C--7 days.

tested application :

Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)

app notes :

Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/5,000 and 1/50,000.

other info1 :

Source: Triosephosphate isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure. Product: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

products categories :

Bacterial, Fungal, Viral

products description :

The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.

ncbi gi num :

145329204

ncbi acc num :

NP_001077931.1

ncbi gb acc num :

NM_001084462.1

uniprot acc num :

Q9SKP6

ncbi mol weight :

33,346 Da

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790118); Biosynthesis Of Amino Acids Pathway (795174); Carbon Fixation In Photosynthetic Organisms Pathway (4065); Carbon Fixation In Photosynthetic Organisms Pathway (395); Carbon Metabolism Pathway (815031); Carbon Metabolism Pathway (817567); Fructose And Mannose Metabolism Pathway (3988); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (825969); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (382683)

ncbi summary :

Encodes a plastidic triose phosphate isomerase. Mutants with reduced pdTPI levels have difficulty transitioning from heterotrophic to autotrophic growth. The related phenotypes, such as chlorosis in light-grown seedlings may result from an accumulation of dihydroxyacetone phosphate (DHAP) and methylglyoxal (MG) in these mutants. Both splice variants appear to be expressed, but the At2g21170.2 variant appears to have a much narrower expression range limited to roots.

uniprot summary :

Catalytic activity: D-glyceraldehyde 3-phosphate = glycerone phosphate. Pathway: Carbohydrate biosynthesis; Calvin cycle. Subunit structure: Homodimer . By similarity. Subcellular location: Plastid chloroplast . By similarity. Miscellaneous: In plants, there are two types of TPIS, cytosolic and plastid. Sequence similarities: Belongs to the triosephosphate isomerase family.

size1 :

10 mg

price1 :

275 USD

more info or order :

MyBioSource product webpage