Goat anti Human properdin | MyBioSource MBS573234 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti Human properdin

catalog :

MBS573234

quantity :

1 mL

price :

290 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS573234

products type :

Antibody

products full name :

Goat anti Human properdin

products short name :

[properdin]

other names :

[properdin; Properdin; properdin; complement factor P; properdin P factor, complement; complement factor properdin; Complement factor P]

other gene names :

[CFP; CFP; BFD; PFC; PFD; PROPERDIN; PFC]

uniprot entry name :

PROP_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human

specificity :

Precipitating polyclonal Goat antiSerum to Human properdin

storage stability :

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 degree C, not refrozen, and preferably used the same day. Lyophilized at +4 degree C--at least 10 years. Reconstituted at or below -20 degree C--3-5 years. Reconstituted at +4 degree C--7 days

tested application :

Immunoprecipitation (IP)

app notes :

Applications: Immunoprecipitation. In immunoelectrophoresis use 2 ul human serum against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 10 ul of the antiserum in 3 mm diameter peripheral wells. In single radial immunodiffusion use 1% antiserum in the gel.

other info1 :

Product type: Primary Antibodies. Source Note: Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure. Product: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

other info2 :

Reactivity Note: The antiSerum does not cross react with any other component of Human plasma. Inter- species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

products categories :

Antisera

products description :

The defined antibody specificity is restricted to properdin as tested at the level of sensitivity of immunoprecipitation techniques. A single precipitin line is obtained in immuno- electrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified properdin. No reaction is obtained with any other plasma protein. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

ncbi gi num :

4505737

ncbi acc num :

NP_002612.1

ncbi gb acc num :

NM_002621.2

uniprot acc num :

P27918

ncbi pathways :

Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865)

ncbi summary :

This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009]

uniprot summary :

CFP: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Defects in CFP are the cause of properdin deficiency (PFD). PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III). Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xp11.4. Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; defense response to bacterium; complement activation, alternative pathway; regulation of complement activation; innate immune response; immune response; post-translational protein modification; complement activation. Disease: Properdin Deficiency, X-linked

size1 :

1 mL

price1 :

290 USD

more info or order :

MyBioSource product webpage