product summary
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company name :
MyBioSource
product type :
antibody
product name :
Rabbit anti Porcine Isocitrate dehydrogenase, conjugated with Biotin
catalog :
MBS573099
quantity :
1 mL
price :
340 USD
clonality :
polyclonal
host :
rabbit
conjugate :
biotin
reactivity :
thale cress
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS573099
products type :
Antibody
products full name :
Rabbit anti Porcine Isocitrate dehydrogenase, conjugated with Biotin
products short name :
Isocitrate dehydrogenase
other names :
isocitrate dehydrogenase; Peroxisomal isocitrate dehydrogenase [NADP]; isocitrate dehydrogenase
other gene names :
ICDH; ICDH; F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
uniprot entry name :
ICDHX_ARATH
clonality :
Polyclonal
host :
Rabbit
reactivity :
Cross-reactivities against enzymes of other sources may occur but have not been determined.
specificity :
Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to isocitRate dehydrogenase from porcine heart.
purity :
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
form :
Biotin-coupled hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
storage stability :
Lyophilized at 4 degree C for at least 10 years. Reconstituted at or below -20 degree C for 3-5 years. Reconstituted at 4 degree C for 7 days.
tested application :
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
app notes :
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as ELISA and Western blotting and immunofluorescence or histochemical techniques). Direction for Use: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/15,000.
other info1 :
Conjugation: Biotin. Preservative: No preservative added, as it may interfere with the antibody activity. No foreign protein added. Immunogen: Isocitrate dehydrogenase isolated and purified from porcine heart. Freund's complete adjuvant is used in the first step of the immunization procedure.
other info2 :
Physicochemical Characteristics: IgG protein concentration 10mg/ml. Biotin/IgG protein molar ration (B/P) approximately 6.5. No foreign proteins added. Marker: N-Hydroxysuccinimidobiotin. Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step, activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
products categories :
Conjugated Secondary Antibody
ncbi gi num :
15221788
ncbi acc num :
NP_175836.1
ncbi gb acc num :
NM_104312.2
uniprot acc num :
Q9SLK0
ncbi mol weight :
47,234 Da
ncbi pathways :
2-Oxocarboxylic Acid Metabolism Pathway (714629); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790118); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (815031); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (3985); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle, First Carbon Oxidation, Oxaloacetate = 2-oxoglutarate Pathway (382688); Citrate Cycle, First Carbon Oxidation, Oxaloacetate = 2-oxoglutarate Pathway (468203)
ncbi summary :
NADP-specific isocitrate dehydrogenase (ICDH)
uniprot summary :
Function: May be involved in response to oxidative stresses . By similarity. Catalytic activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Cofactor: Binds 1 magnesium or manganese ion per subunit . By similarity. Subcellular location: Peroxisome Ref.5 Ref.6. Sequence similarities: Belongs to the isocitrate and isopropylmalate dehydrogenases family.
size1 :
1 mL
price1 :
340 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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