catalog number :
MBS573076
products full name :
Rabbit anti Chicken ovalbumin
products short name :
[ovalbumin]
other names :
[ovalbumin]
reactivity :
Inter-species cross reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
specificity :
A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilution of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum.
form :
Delipidate, heat, inactivated, lyophilized,stable whole serum.
storage stability :
Lyophilized at 4°C: at least 10 years. Reconstituted at or below -20°C: 3-5 years. Reconstituted at 4°C: 7 days. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
tested application :
Precipitation assays
app notes :
Precipitin titre not less than 1:16 when tested against an 1% solution of chicken egg albymin in agar-block immunodifussion titration.
other info1 :
Preservative: No preservative added. Immunogen: Highly purified egg albumin (MW 45,000)isolated from chicken egg whites. Freund's complete adjuvants is used in the first step of the immunization procedure. Protein Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum.No foreign proteins added. Intended use: In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion( Mancini, Ouchterlony) to identify the presence of chicken ovalbumin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
products categories :
Antisera
ncbi acc num :
AAB59956.1
uniprot summary :
ovalbumin: Non-inhibitory serpin. Storage protein of egg white. Belongs to the serpin family. Ov-serpin subfamily. Cellular Component: extracellular space; extracellular region; phagocytic vesicle; cytosol. Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding