product summary
Loading...
company name :
MyBioSource
product type :
antibody
product name :
Rabbit anti Collagen IV
catalog :
MBS573001
quantity :
0.25 mL
price :
265 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS573001
products type :
Antibody
products full name :
Rabbit anti Collagen IV
products short name :
Collagen IV
clonality :
Polyclonal
host :
Rabbit
reactivity :
Bovine, Human, Rat, Swine
specificity :
Polyclonal type IV collagen recognizes the extracellular - basement membranes.
form :
Each vial contains 250ul polyclonal Serum including 0.09% sodium azide
storage stability :
Store at 4 degree C, or in small aliquots for prolonged storage at -20 degree C.
tested application :
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
app notes :
Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room tempeRature. Optimal antibody dilution should be determined by titRation; 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
other info1 :
Source Note: This polyclonal type IV collagen antibody is a Rabbit polyclonal antibody. The Rabbits were immunized with isolated Human type IV collagen. The immunogen has been isolated from Human placenta.
products description :
The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiSerum is important in the differential diagnosis of bullous lesions in dermatopathology.
products references :
Visser, R., van der Beek, J.M.H., Havenith, M.G., Cleutjens, J.P.M. and Bosman, F.T. (1985). Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia. Histopathology 10, 171-180. Cleutjens, J.P.M., Havenith, M.G., Kasper, M., Vallinga, M., and Bosman, F.T. (1990). Absence of type IV collagen in the centre of the corneal epithelial basement membrane. Histochemical Journal 22, 688-94. Havenith, M.G. et al. (1987). Human specific anti-type IV collagen monoclonal antibodies, characterisation and immunohistochemical appliCation. Histochemistry 87, 123-28. Schapers, R.F.M., Pauwels, R.P.E., Havenith, M.G., Smeets, A.W.G.B, van den Brandt and P.A. Bosman, F.T. (1990). Prognostic Significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder. Cancer 66, 2583-88.
size1 :
0.25 mL
price1 :
265 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!