secondary antibody

Goat Ig anti Human IgA (Fc specific) immunofixation

MBS571716

1 mL

275 USD

Secondary Antibody

Goat Ig anti Human IgA (Fc specific) immunofixation

Goat Ig anti Human IgA (Fc specific) immunofixation

Polyclonal

Goat

Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations o

The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses stric isotypic (class) specificity. It does not react with any non-Ig protein human serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG (7S) fraction by DEAE-chromatography.

Goat Ig lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Packing: Vial with 1 mllyophilized Ig fraction. Storage I shelf life:. Lyophilized at +4 degree C = at least 10 years. reconstituted at or below -20 degree C = 3-5 years. reconstituted at +4 degree C =7 days

Immunoprecipitation (IP)

Preservative: No preservative added, as it may interfere with the antibody activity. Immunogen: Highly purified polyclonal IgA and monoclonal IgA2 isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure. Physicochemical characteristic: IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre not less than 1 :64 when tested against normal human serum in agar immunodiffusion block titration. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has not been tested for cross-reactivity. Caution: This product should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only.

Human immunoglobulins

This antiserum is intended to detect and identify IgA in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

1 mL

275 USD