secondary antibody

Rabbit anti Monkey IgG Fab, conjugated with Horseradish peroxidase

MBS571697

1 mL

425 USD

Secondary Antibody

Rabbit anti Monkey IgG Fab, conjugated with Horseradish peroxidase

Rabbit anti Monkey IgG Fab

Polyclonal

Rabbit

Monkey

Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Monkey Fab of IgG. The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both y:, light chain types, with their Fab or F(ab')2 subunits, and with free light chains of kappa and lambda type In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled monkey serum a characteristic precipitin line IS obtained, which shows a reaction of identity with the precipitin line of the purified Fab.

Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution In phosphate buffered saline (PBS, pH 7.2).

Handling: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove Insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Packing: Vial with 1 mllyophilized immunoconjugate. Storage & Shelf Life:. Lyophilized at +4°C at least 10 years. reconstituted at or below -20°C 3-5 years. reconstituted at +4°C 7 days

ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Dot Blot (DB), Immunoblotting (IB)

Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staming and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1: 1 00 and 1 :500; in ELISA and comparable non-precipitating antibody-binding assays between 1 :5,000 and 1: 1 0,000.

Product Type: Secondary Antibodies. Source: Purified normal Fab of polyclonal rhesus monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure. Product: Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with he antibody activity. Conjugation: HRP. Preservative: No preservative added, as it may interfere with he antibody activity. Immunogen: Purified normal Fab of polyclonal rhesus monkey IgG. Freund's complete adjuvant is used in the first step of the immunization procedure. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a reaction has been obtained with Human immunoglobulins. Physicochemical &characteristics: IgG protein concentration 10 mg/ml. Peroxidase/lgG protein molar ratio (E/P) is approximately 1.7 No foreign proteins added. Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ=3.2). Conjugation procedure: Conjugation is carried out using a proprietary modification of the period ate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.Caution: This immunoconjugate should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only.

Conjugated Secondary Antibody

The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled monkey serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

1 mL

425 USD