secondary antibody

Rabbit anti pigeon IgG (heavy and light chains), conjugated with Biotin

MBS571625

1 mL

275 USD

Secondary Antibody

Rabbit anti pigeon IgG (heavy and light chains), conjugated with Biotin

Rabbit anti pigeon IgG (heavy and light chains)

Polyclonal

Rabbit

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the con

Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to pigeon IgG, heavy and light chains. he reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common F ab portion. II does not react with any non-Ig protein in pigeon serum, as tested by immunoelectrophoresis and double radial immuno-diffusion.

Hyperimmune antisera with sirong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromalography.

Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Handling: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Storage I shelf life:. lyophilized at +4 degree C = at least 10 years . reconstituted at or below -20 degree C =3-5 years . reconstituted at +4 degree C=7 days . Caution: This immunoconjugate should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only

ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)

Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500; . in ELISA and comparable non-precipitating antibody-binding assays between 1 :2,000 and 1 :15,000.

Conjugation: Biotin. Preservative: No preservative added. Immunogen: Purified normal IgG isolated from pooled pigeon serum. Freund's complete adjuvant is used in the first step of the immunization procedure. Adsorption: No adsorption required. Physicochemical characteristics : IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) is approximately 3.3. No foreign proteins added. Marker: N-Hydroxysuccinimidobiotin. Conjugation procecture: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Intended use: In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of pigeon origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentRation of the reactants and the sensitivity of the assay format. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a reaction with Chicken, Duck and Turkey has been observed. Caution: This immunoconjugate should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only

Conjugated Secondary Antibody

1 mL

275 USD