secondary antibody

Goat anti Dog IgA (Fc specific)

MBS571617

1 mL

290 USD

Secondary Antibody

Goat anti Dog IgA (Fc specific)

[Goat anti Dog IgA (Fc specific)]

Polyclonal

Goat

Dog

Precipitating polyclonal Goat antiSerum to Dog IgA, Fc specific

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Precipitation assays.

Precipitation assays. In immunoelectrophoresis use 2 ?l serum or equivalent against 120 ?l antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ?l antiserum in 3 mm diameter center well and 2 ?l serum samples (neat and serially diluted in 2 mm diameter peripheral wells

Host Note: Highly purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. Form Note: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.

Reactivity Note: The antiSerum does not cross-react with any other component of the Dog immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Antisera, Veterinary

The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal dog serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgA from serum or plasma.

1 mL

290 USD