secondary antibody

Goat anti Horse IgM (Fc specific)

MBS571482

1 mL

265 USD

Secondary Antibody

Goat anti Horse IgM (Fc specific)

Goat anti Horse IgM (Fc specific)

Polyclonal

Goat

The antiserum does not cross-react with any other component of the horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivit

The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal horse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments.

Delipidated, heat inactivated, lyophilized, stable whole antiserum

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Packing:Vial with 1 ml lyophilized antiserum. Lyophilized at +4 degree C = at least 10 years. reconstituted at or below -20 degree C =3-5 years. reconstituted at +4 degree C = 7 days. Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.

Immunoprecipitation (IP)

Preservative: No preservative added. Immunogen: Highly purified normal IgM isolated from pooled horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure. Absorption: Immunoaffinity adsorbed using in solubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevent the presence of excess adsorbent protein or immune complexes in the antiserum. Protein Concentrion: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody Titre: Precipitin titre 1 :32 when tested against pooled normal horse serum in agar-block immunodiffusion titration. Direction of use: In immunoelectrophoresis use 2 uL or equivalent against 120 uL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 uL in a 3 mm diameter center well and 2 uL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Reactivity Note: The antiSerum does not cross-react with any other component of the Horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Antisera, Veterinary

In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma.

1 mL

265 USD