secondary antibody

Goat anti Human IgG IgA IgM (Fc specific), conjugated with Horseradish peroxidase

MBS571179

1 mL

340 USD

Secondary Antibody

Goat anti Human IgG IgA IgM (Fc specific), conjugated with Horseradish peroxidase

Goat anti Human IgG IgA IgM (Fc specific)

Polyclonal

Goat

Human. Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum with other spec

Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Human IgG, IgA and IgM, Fc specific

The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodi

Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Store at 4 degree C. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight preCipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Packing:Vial with 1 ml lyophilized immunoconjugate. Storage I shelf life:. Lyophilized at +4°C at least 10 years . reconstituted at or below -20°C 3-5 years. reconstituted at +4°C 7 days

ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)

Caution: This immunoconjugate should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only

Preservative: No preservative added, as it may interfere with the antibody activity. Immunogen: Purified polyclonal human IgG, and IgA and IgM containing factions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure. Adsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted human serum fractions and IgG/Fab as required to eliminate antibodies reacting with the common Fab portion of immunoglobulins or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Identity & Specificity: The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the human immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Physicochemical characteristics: IgG protein concentration 10mg/ml. Peroxidase/lgG protein molar ratio (E/P) is approximately 1.5. No foreign proteins added. Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ=3.2). Conjugation procedure: Conjugation is carried out (Ising a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.

Product Specificity Group: Antisera to immunoglobulins (Ig), Ig subunits and structurally related polypeptides are raised against total Ig and specific Ig subtypes purified from serum or appropriate secretions. Reagents intended for the detection of human antigens or antigens from other species, are cross adsorbed to meet the additional specificity criteria. Polyclonal antisera to subclasses of human Ig are raised against purified myeloma proteins and made specific by cross adsorption. They are analvsed for specificity and titer in special Immunodiffusion and immunoelectrophoresis tests. Antisera are also available for human and animal secretory proteins, components of the complement system, as well as coagulation factors and related proteins. Monoclonal antibodies include labelled and unlabelled monoclonal antibodies of murine and rat origin. Many of these highly specific, affinity purified immunoreagents have been published extensively. They are directed against immunoglobulin subtypes on the one hand, and cell and tissue specific C'1olecules on the other. These reagents are widely used in biomedical and veterinary research, amongst others in immunology, virology, oncology, cardiovascular disease and neuropathology. The quality control of these reagents includes amongst others immunocytochemistry on cell lines, immunohistochemical staining of tissue sections and immunoblotting. Detailed product specificatons are available, and batch specific information will be provided with the reagents. In addition to resarch reagents for in vitro studies on human and laboratory animal systems, specific antisera and immunoconjugates are available for use in studies of domestic and farm animal species. Antibody titres: The potency or strength of an antiserum is commonly expressed as its titre, a figure referring to the endpoint of an immunological test. Polyclonal antisera always react with different antigenic determinants for which they have a variable affinity under different test conditions. Only part of the antibodies present will contribute to the test result under the given test conditions. Precipitating antibodies may not participate in non-precipitating antibody binding techniques and vice versa. Consequently, the titre of an antiserum is not a generally valid and constant characteristic. The titre of a cytochemical or immunoassay grade reagent can be expressed as the optimum working dilution in a performance test. It can only serve as a general guideline since it varies with the test conditions. Caution and Warning: Immunological products and all associated materials should be handled by qualified personel and appropriate precautions should be taken in their handling and disposal. They are intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. The products may contain sodium aZide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.

Conjugated Secondary Antibody

In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.

1 mL

340 USD