catalog number :
MBS571036
products type :
Secondary Antibody
products full name :
Mouse anti Human IgG2 (Fab subclass specific)
products short name :
[Mouse anti Human IgG2 (Fab subclass specific)]
clone :
[NI 6014 (HP 6014)]
specificity :
Monoclonal Mouse antibody to Human IgG2, subclass specific.
form :
Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added.
storage stability :
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day.
tested application :
Indirect Immunofluorescence (IF), ELISA (EIA)
other info1 :
Host Note: Highly purified monoclonal IgG2 isolated from pooled human serum . Reconstitution: Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
other info2 :
Reactivity Note: The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
products categories :
Human immunoglobulins
products description :
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.