Mouse anti actin alpha-skeletal | MyBioSource MBS570258 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti actin alpha-skeletal

catalog :

MBS570258

quantity :

0.1 mg

price :

415 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[3B3]

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

image

image 1 :

Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 (1:250)

image 2 :

product information

catalog number :

MBS570258

products type :

Antibody

products full name :

Mouse anti actin alpha-skeletal

products short name :

[actin alpha-skeletal]

other names :

[actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; nemaline myopathy type 3; actin, alpha 1, skeletal muscle; Alpha-actin-1]

other gene names :

[ACTA1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM; ACTA]

uniprot entry name :

ACTS_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[3B3]

host :

Mouse

reactivity :

Caprine, Goat, Human, Rabbit, Rat, Swine.

specificity :

Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.

form :

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

storage stability :

The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

tested application :

ELISA, Immunoblotting, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blotting.

app notes :

3B3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Source Note: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.

other info2 :

Reactivity Note: The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.

products categories :

Cytoskeletal, Cardiovascular, Cell differentiation, Cytoskeleton, Stem cell research

products description :

Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.

products references :

Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.

ncbi gi num :

4501881

ncbi acc num :

NP_001091.1

ncbi gb acc num :

NM_001100.3

uniprot acc num :

P68133

ncbi mol weight :

42,051 Da

ncbi pathways :

Caspase Cascade In Apoptosis Pathway (137974); Hypothetical Network For Drug Addiction Pathway (198776); Myometrial Relaxation And Contraction Pathways (198759); RhoA Signaling Pathway (169356); Signaling Events Mediated By Focal Adhesion Kinase Pathway (169348); Striated Muscle Contraction Pathway (198903)

ncbi summary :

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

uniprot summary :

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family. Protein type: Cell development/differentiation; Contractile; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q42.13. Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton. Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding. Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding. Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion

size1 :

0.1 mg

price1 :

415 USD

more info or order :

MyBioSource product webpage