antibody

Mouse anti T-Cell Receptor gamma delta

MBS570229

0.1 mg

415 USD

monoclonal

mouse

nonconjugated

[11F2]

ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, enzyme immunoassay, immunohistochemistry - frozen section

Antibody

Mouse anti T-Cell Receptor gamma delta

[T-Cell Receptor gamma delta]

Monoclonal

IgG1

[11F2]

Mouse

Human

Clone 11F2 recognizes all molecular forms of the gamma/delta T-cell receptor (TCR) desribed so far.

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Store at 4°C, or in small aliquots at -20°C.

ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP)

11F2 is useful for Enzyme Linked Immuno Sorbent Assays, Flow Cytometry, Immunohistochemistry on frozen sections and Immunoprecipitation. Optimal antibody dilution should be determined by titRation.

Product Type: Primary Antibodies. Source: 11F2 (anti-TCR gamma delta-l) is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Balb/c mice immunized with protein A-Sepharose/anti-CD3/antigen complex.

CD markers, Cell adhesion, Immunology

Two types of T cell antigen receptors (TCRs) are now known, the alpha/beta, and the gamma/delta heterodimer, which are expressed at the cell surface in association with the CD3 molecular complex. TCR-gamma can occur in either disulphide-linked or non-disulphide-linked forms. The gamma/delta T-cell receptor is present on a minor subset of T lymphocytes in peripheral blood, thymus, spleen, and lymph node. In man and Mouse, only a small part of the T lymphocytes of the peripheral blood ( 5%) is gamma/delta. gamma/delta T lymphocytes may complement alpha/beta T cells in terms of antigenic specificities, functional capabilities, or sites of action within the body.

Borst, J., Van Dongen, J., Bolhuis, R., Peters, P., Hafler, D., De Vries, E., Van de Griend, R. (1988). Distinct molecular forms of Human T cell receptor g/d detected on viable T cells by a monoclonal antibody. I. Exp. Med 167 1626-1644 Vroom, T., Scholte, G., Ossendorp, F., Borst, J. (1991). Tissue distribution of Human g/d T cells: No evidence for general epithelial tropism. J. Clon. Pathol. 44 1012-1017 Bos, J.D., Teunissen, M.B.M., Cairo, I., Krieg, S.R., Kapsenberg, M.L., Das, P.K., and Borst, J. (1990). T-cell receptor gamma delta bearing cells in normal Human skin J. Invest. Dermatol. 94: 37-42. Lanier, L., Ruitenberg, J., Bolhuis, R., Borst, J., Phillips, J., Testi, R. (1988). Structural and serological heterogeneity of g/d T cell antigen receptor expression in thymus and peripheral blood. Eur J Immunol. 18 1985-1992 Lanier, L, Serafini, A., Ruitenberg, J., et all. (1987) The gamma T-cell antigen receptor. J Clin Immunol. 7 429 Testi, R., Lanier, L. (1989). Functional expression of CD28 on T cell antigen receptor g/d-bearing T lymphocytes. Eur J Immunol 19 185 Lanier, L., Federspiel, N., Ruitenberg, J., Phillips, J., Allison, J., Weiss, A. (1987(. The T cell antigen receptor complex expressed on normal peripheral blood CD4--, CD8- T lymphocytes: A CD3-associated disulfide-linked g chain heterodimer. J Exp Med 165 1076-1094. Voogt, P., Falkenburg, J., Fibbe, W., et al. (1989). Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor-/CD3-, T cell receptor gd+/CD3+ and T cell receptor-alpha/beta+/CD3+ lymphocytes 142 1774-1780.

0.1 mg

415 USD