antibody

Mouse anti Vimentin, conjugated with FITC

MBS570210

1 ml

370 USD

monoclonal

mouse

FITC

RV202

human, mouse, rat, dog, chicken, zebrafish

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Antibody

Mouse anti Vimentin, conjugated with FITC

Vimentin

vimentin; Vimentin; vimentin; vimentin

VIM; VIM; CTRCT30

VIME_HUMAN

Monoclonal

IgG1

RV202

Mouse

Canine, Chicken, Caprine, Human, Monkey, Mouse, Rat, Swine, Zebrafish

RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, immuno-histochemistry on frozen tissues, as well as for flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:10.

Each vial contains 1ml FITC-conjugated vimentin monoclonal antibody in PBS containing 0,1% BSA, 0.09% sodium azide. Approximately 100 tests.

Store at 4 degree C, or in small aliquots at -20 degree C.

Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)

RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

Conjugation: FITC. Source Note: RV202 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal vimentin extract of calf lens.

Cytoskeletal, Tumour marker, Cancer, Cytoskeleton, Immunology, Neurobiology, Stem cell research

Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of keRatin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. Viebahn, C., Lane, E. B., and Ramaekers, F. C. (1988). KeRatin and vimentin expression in early organogenesis of the Rabbit embryo, Cell Tissue Res 253, 553-62. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.

62414289

NP_003371.2

NM_003380.3

P08670

53,652 Da

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Aurora B Signaling Pathway 138080!!Caspase Cascade In Apoptosis Pathway 137974!!Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway 105679!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!MicroRNAs In Cancer Pathway 852705

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Ref.37Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Ref.37. Subunit structure: Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) . By similarity. Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B . By similarity. Ref.23 Ref.27 Ref.32 Ref.37 Ref.42. Subcellular location: Cytoplasm Ref.39. Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17. Domain: The central alpha-helical coiled-coil rod region mediates elementary homodimerization. Ref.42. Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin . By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Ref.14 Ref.19 Ref.20 Ref.21 Ref.27 Ref.39. Sequence similarities: Belongs to the intermediate filament family. Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.

1 ml

370 USD