antibody

Mouse anti Integrin beta 1D

MBS570131

0.1 mg

330 USD

monoclonal

mouse

nonconjugated

1G2

human, mouse

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

Antibody

Mouse anti Integrin beta 1D

Integrin beta 1D

integrin beta-1 isoform 1D; Integrin beta-1; integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta

ITGB1; ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; FNRB; MDF2; MSK12; GPIIA

ITB1_HUMAN

Monoclonal

IgG2a

1G2

Mouse

Human, Mouse

1G2 recognizes specifically the cytoplasmic domain of integrin subunit beta1D present in cardiac and skeletal muscle.

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

1 mg/ml

Store at 4 degree C, or in small aliquots at -20 degree C.

Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)

1G2 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting appliCations.

Source Note: 1G2 is a Mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin beta1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.

Reactivity Note: A broad species reactivity is expected because of the conserved nature of the epitope.

Cell adhesion, Cardiovascular, Cell adhesion

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migRation and apoptosis. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. beta1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.

van der Flier, A., Gaspar, A. C., Thorsteinsdottir, S., Baudoin, C., Groeneveld, E., Mummery, C. L., and Sonnenberg, A. (1997). Spatial and temporal expression of the beta1D integrin during Mouse development, Dev Dyn 210, 472-86.

19743819

NP_391988.1

NM_033668.2

P05556

88,415 Da

Adaptive Immune System Pathway (366160); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807)

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle). Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Cell surface; Receptor, misc. Chromosomal Location of Human Ortholog: 10p11.2. Cellular Component: cell surface; focal adhesion; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; melanosome; plasma membrane; basement membrane; neuromuscular junction; integrin complex; receptor complex; sarcolemma; cleavage furrow; filopodium; external side of plasma membrane. Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; alpha-actinin binding; actin binding; protein kinase binding; peptide binding; integrin binding; viral receptor activity; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding. Biological Process: extracellular matrix organization and biogenesis; maternal process involved in pregnancy; regulation of cell cycle; positive regulation of apoptosis; axon extension; mesodermal cell differentiation; positive regulation of endocytosis; cardiac muscle cell differentiation; leukocyte adhesion; transforming growth factor beta receptor signaling pathway; germ cell migration; tissue homeostasis; response to drug; dendrite morphogenesis; protein transport within lipid bilayer; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of neuron differentiation; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; response to activity; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; axon guidance; entry of virus into host cell; cell-matrix adhesion; cell fate specification; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; cell migration; regulation of immune response; in utero embryonic development; sarcomere organization; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; cellular defense response; leukocyte tethering or rolling; homophilic cell adhesion; blood coagulation; positive regulation of cell migration

0.1 mg

330 USD