Rat anti E-selectin | MyBioSource MBS570119 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat anti E-selectin

catalog :

MBS570119

quantity :

0.1 mg

price :

325 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

UZ4

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

product information

catalog number :

MBS570119

products type :

Antibody

products full name :

Rat anti E-selectin

products short name :

E-selectin

other names :

E-selectin; E-selectin; E-selectin; ELAM-1; OTTHUMP00000032745; OTTHUMP00000032746; OTTHUMP00000032747; OTTHUMP00000032748; OTTHUMP00000032749; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; selectin E; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2

other gene names :

SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1

uniprot entry name :

LYAM2_HUMAN

clonality :

Monoclonal

isotype :

IgM

clone :

UZ4

host :

Rat

reactivity :

Human, Mouse

specificity :

UZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectin domain of both murine and Human E-selectin.

form :

Each vial contains 100ul 1 mg/ml purified monoclonal antibody in PBS containing 0,09% sodium azide.

storage stability :

Store at 4 degree C, or in small aliquots at -20 degree C.

tested application :

Flow Cytometry (FC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

app notes :

UZ4 is suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry (dilution 1:500). To analyze E-selectin protein in frozen sections we refer to the anti E-selectin monoclonal antibodies UZ5, UZ6, and UZ7. For a functional UZ4 antibody that inhibits leucocyte adhesion to murine and Human activated endothelial cells refer to the E-selectin antibody for in vivo use which does not contain azide.

other info1 :

Source Note: UZ4 is a Rat monoclonal IgM antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

products categories :

Cell adhesion, Cell adhesion, Immunology

products description :

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkappaB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

products references :

Hallmann, R., Zimmermann, U., Sorokin, L. M., Needham, L., and Von der Mark, K. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. Austrup, F., Vestweber, D., Borges, E., Lohning, M., Brauer, R., Herz, U., Renz, H., Hallmann, R., Scheffold, A., Radbruch, A., and Hamann, A. (1997). P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues, Nature 385, 81-3. Borges, E., Tietz, W., Steegmaier, M., Moll, T., Hallmann, R., Hamann, A., and Vestweber, D. (1997). P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migRation into inflamed skin, J Exp Med 185, 573-8. Tietz, W., Allemand, Y., Borges, E., von Laer, D., Hallmann, R., Vestweber, D., and Hamann, A. (1998). CD4+ T cells migRate into inflamed skin only if they express ligands for E- and P-selectin, J Immunol 161, 963-70. Alferink, J., Tafuri, A., Vestweber, D., Hallmann, R., Hammerling, G. J., and Arnold, B. (1998). Control of neonatal tolerance to tissue antigens by peripheral T cell trafficking, Science 282, 1338-41. Wolf, D., Hallmann, R., Sass, G., Sixt, M., Kusters, S., Fregien, B., Trautwein, C., and Tiegs, G. (2001). TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis, J Immunol 166, 1300-7. Hammel, M., Weitz-Schmidt, G., Krause, A., Moll, T., Vestweber, D., Zerwes, H. G., and Hallmann, R. (2001). Species-specific and conserved epitopes on Mouse and Human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74. Sunderkotter, C., Seeliger, S., Schonlau, F., Roth, J., Hallmann, R., Luger, T. A., Sorg, C., and Kolde, G. (2001). Different pathways leading to cutaneous leukocytoclastic vasculitis in mice, Exp Dermatol 10, 391-404. Kneilling, M., Reinhard, M., Hültner, L., Schönberger, T., Fuchs, K., Schaller, M., Bukala, D., Massberg, S., Sander, C.A., Braumüller, H., Eichner, M., Maier, K.L., Hallmann, R., Pichler, B.J., Haubner, R., Gawaz, M., Pfeffer, K., Biedermann, T., Röcken, M. (2009). Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation. Blood, 114, 1696-1706.

ncbi gi num :

187960042

ncbi acc num :

NP_000441.2

ncbi gb acc num :

NM_000450.2

uniprot acc num :

P16581

ncbi mol weight :

66,655 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway 138006!!African Trypanosomiasis Pathway 194384!!African Trypanosomiasis Pathway 194323!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!Malaria Pathway 152665!!Malaria Pathway 152657!!Thromboxane A2 Receptor Signaling Pathway 138060

ncbi summary :

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq]

uniprot summary :

Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Ref.1. Subunit structure: Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction. Ref.9 Ref.12. Subcellular location: Membrane; Single-pass type I membrane protein. Polymorphism: A polymorphism in position 149 is associated with a higher risk of coronary artery disease (CAD). A significantly higher mutation frequency (Arg-149) is observed in patients with angiographically proven severe atherosclerosis compared with an unselected population (Ser-149). Sequence similarities: Belongs to the selectin/LECAM family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 6 Sushi (CCP/SCR) domains.

size :

0.1 mg

price :

325 USD